FastQCFastQC Report
Tue 26 Sept 2023
SRR23951077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23951077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84610279
Total Bases12.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA985761111.65060689611956No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTTGGAATTCTCGGGTGCCAA43379905.127024814561834No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCTGGAATTCTCGGGTGCCAA43354745.124051180590008No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC12029221.4217208762542906No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA11764001.3903748030425476No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA10227971.20883303079523No Hit
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC8594081.0157252879404877RNA PCR Primer, Index 1 (100% over 26bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCA6854580.8101356101189549No Hit
TGGCTGTAGTTTAGTGGTTAGAATTCCACGTTGGAATTCTCGGGTGCCAA5307220.6272547570727193No Hit
TTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAG4950470.5850908493044917No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTTGGAATTCTCGGGTGCCA4745350.5608479319634438No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTGGAATTCTCGGGTGCCAA4373300.5168757332664037No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA4045450.47812748614148876No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCTGGAATTCTCGGGTGCCA4030430.47635228811856295No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTGGAATTCTCGGGTGCC3605620.4261444404408595No Hit
GCCCTTTTAACTCAGTGGTAGAGTAACGCCTGGAATTCTCGGGTGCCAAG3302930.39036982728776964No Hit
CTTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAA2985620.3528672916915922No Hit
CGCGGGGTAGAGCAGTTTGGTAGCTCGCAAGGCTGGAATTCTCGGGTGCC2626080.310373636753993No Hit
TTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAG2591410.30627602587151376No Hit
TGCCGGCCGGGGGACGGACTGGGAATTCCCCGGGCTGGAATTCTCGGGTG2550420.3014314608275905No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTTGGAATTCTCGGGTGCC2501370.29563429284992665No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC2495310.2949180678153774No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTGGAATTCTCGGGTGCCAAG2377970.2810497764698306No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGGAATTCTCGGGTGCCA2207880.26094701803311626No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAGG2073160.2450246027435981Illumina Small RNA Adapter 2 (100% over 21bp)
GGGTTGCTAACTCAACGGTAGAGTACTCGGCTGGAATTCTCGGGTGCCAA2033380.24032304632868542No Hit
CCCTGGTTGATGAACCATGACCGTGCGGCTTGGAATTCTCGGGTGCCAAG1919440.22685659741176364No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTGGAATTCTCGGGTGCCAA1876480.221779200137137No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTTGGAATTCTCGGGTGCCA1662020.1964323980068663No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC1544810.18257947122476692No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA1472870.17407695819086No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTGGAATTCTCGGGTGCCA1439970.170188541749165No Hit
TCCGATGTCGTCCAGCGTTAGGATATCTGGCTGGAATTCTCGGGTGCCAA1429320.16892982943597196No Hit
TGCCGGCCGGGGGACGGACTGGGAACTGGAATTCTCGGGTGCCAAGGAAC1352660.15986946455997386RNA PCR Primer, Index 1 (100% over 24bp)
GTGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACT1249600.14768891141465212RNA PCR Primer, Index 1 (100% over 25bp)
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCTGGAATTCTCGGGTGCCAA1221350.14435007358857663No Hit
AGGGATATAACTCAGCGGTAGAGTGTCACCTGGAATTCTCGGGTGCCAAG1219440.14412433269484903No Hit
GGGTCCATAGCTCAGTGGTAGAGCATTTGATGGAATTCTCGGGTGCCAAG1216470.14377331151455014No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG1176150.13900793306685585No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTTGGAATTCTCGGGTGC1126990.13319776430473654No Hit
TAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTGGAATTCTCGGGTGC1033460.1221435518490608No Hit
TGGCCTGCTGGTCCGATCTCATCTGAACCACCTGGAATTCTCGGGTGCCA968350.11444826934089178No Hit
TGCCGGCCGGGGGACGGACTGGGAATGGAATTCTCGGGTGCCAAGGAACT924680.10928695791205226RNA PCR Primer, Index 1 (100% over 25bp)
TGGCTGTAGTTTAGTGGTTAGAATCCCACGTTGGAATTCTCGGGTGCCAA887720.10491869433499917No Hit
GCACCAGTAGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG871910.10305012704189287No Hit
GGTCGTAGGTTCGAATCCTACTTGGGGATGGAATTCTCGGGTGCCAAGGA867330.10250882165274504RNA PCR Primer, Index 1 (100% over 22bp)
TTTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAA846670.10006703795410012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)