FastQCFastQC Report
Mon 25 Sept 2023
SRR23951078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23951078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52867492
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA581996711.008592955383623No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTTGGAATTCTCGGGTGCCAA26102774.9373951766049355No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCTGGAATTCTCGGGTGCCAA23497124.444530865962017No Hit
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC11637192.201199557565545RNA PCR Primer, Index 1 (100% over 26bp)
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC9282541.7558124376318058No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA7537501.4257343624320216No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA6394731.2095769551542184No Hit
TTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAG3919280.7413402549907229No Hit
TGGCTGTAGTTTAGTGGTTAGAATTCCACGTTGGAATTCTCGGGTGCCAA3827340.723949605931751No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCA3782940.7155512502843903No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA2626820.4968686617477523No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTGGAATTCTCGGGTGCCAA2479390.46898195965112166No Hit
CTTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAA2344430.44345398491761245No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTTGGAATTCTCGGGTGCCA2268710.4291313838000865No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC2103780.3979345190046087No Hit
GCCCTTTTAACTCAGTGGTAGAGTAACGCCTGGAATTCTCGGGTGCCAAG1933540.3657332562702237No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTGGAATTCTCGGGTGCC1787330.33807731980174127No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTTGGAATTCTCGGGTGCC1564550.295938002884646No Hit
TTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAG1553940.2939310985283735No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCTGGAATTCTCGGGTGCCA1477000.2793777317826993No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGGAATTCTCGGGTGCCA1436280.2716754560628675No Hit
CCCTGGTTGATGAACCATGACCGTGCGGCTTGGAATTCTCGGGTGCCAAG1418370.2682877409807902No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAGG1406310.266006566000899Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC1282510.2425895293084832No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTGGAATTCTCGGGTGCCAAG1242300.2349837211873035No Hit
GTGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACT1145940.21675701960668006RNA PCR Primer, Index 1 (100% over 25bp)
TGCCGGCCGGGGGACGGACTGGGAATTCCCCGGGCTGGAATTCTCGGGTG1122860.2123913878872862No Hit
TCCGATGTCGTCCAGCGTTAGGATATCTGGCTGGAATTCTCGGGTGCCAA1104000.20882397825870008No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG1103340.20869913783691496No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTGGAATTCTCGGGTGCCA1078920.20408004223086657No Hit
CGCGGGGTAGAGCAGTTTGGTAGCTCGCAAGGCTGGAATTCTCGGGTGCC992030.18764461155070492No Hit
GGGTTGCTAACTCAACGGTAGAGTACTCGGCTGGAATTCTCGGGTGCCAA930060.17592285255370163No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGC916400.17333903412705864RNA PCR Primer, Index 27 (100% over 50bp)
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCTGGAATTCTCGGGTGCCAA879370.1663347298562981No Hit
TGCCGGCCGGGGGACGGACTGGGAACTGGAATTCTCGGGTGCCAAGGAAC863900.1634085460305172RNA PCR Primer, Index 1 (100% over 24bp)
GGGTCCATAGCTCAGTGGTAGAGCATTTGATGGAATTCTCGGGTGCCAAG776120.1468047699330999No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTTGGAATTCTCGGGTGCCA751020.14205705086218198No Hit
GCACCAGTAGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG729710.13802621845575727No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTGGAATTCTCGGGTGCCAA708340.13398403692007935No Hit
TGCCGGCCGGGGGACGGACTGGGAATGGAATTCTCGGGTGCCAAGGAACT692570.13100110744803253RNA PCR Primer, Index 1 (100% over 25bp)
TGGCCTGCTGGTCCGATCTCATCTGAACCACCATGGAATTCTCGGGTGCC683630.12931008718930717No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA676350.12793305950658676No Hit
AGGGATATAACTCAGCGGTAGAGTGTCACCTGGAATTCTCGGGTGCCAAG654750.1238473729754383No Hit
TGGCCTGCTGGTCCGATCTCATCTGAACCACCTGGAATTCTCGGGTGCCA648050.12258005354216538No Hit
TGGCTGTAGTTTAGTGGTTAGAATCCCACGTTGGAATTCTCGGGTGCCAA643820.12177993992981548No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTTGGAATTCTCGGGTGC621010.117465379292061No Hit
AACAGAAAAGATAACTCTTCCCGAGGCCCCCGCTGGAATTCTCGGGTGCC604210.11428762310116773No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC599720.11343832992872066No Hit
TCGGGGGTCCCAGTTCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAAG590350.11166597424368079No Hit
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA584750.11060672218004969RNA PCR Primer, Index 1 (100% over 34bp)
TCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTTGGAATTCTCGGGTGC583740.11041567850428767No Hit
AGAGCCCCGTCGTGCCCGGACCCTGTCGCATGGAATTCTCGGGTGCCAAG583210.11031542786255114No Hit
AGAAGCGTCCTCAGCGACGGACCGGGCCTGGAATTCTCGGGTGCCAAGGA571830.10816287634752941RNA PCR Primer, Index 1 (100% over 22bp)
TTGGAACCCTGAACAGACTGCCGGTTGGAATTCTCGGGTGCCAAGGAACT551870.10438739934930145RNA PCR Primer, Index 1 (100% over 25bp)
TTTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAA542130.10254505736720025No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)