FastQCFastQC Report
Mon 25 Sept 2023
SRR23951085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23951085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60991459
Total Bases9.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA24859784.0759444695363No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTTGGAATTCTCGGGTGCCAA7006421.1487542870551761No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTTGGAATTCTCGGGTGCC6845441.1223604275477326No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCTGGAATTCTCGGGTGCCAA5180400.8493648266390872No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA4154290.6811265164192908No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCA4088970.6704168201649349No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC3841320.6298127742771328No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA3027420.49636786029335683No Hit
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC2126580.34866849143582546RNA PCR Primer, Index 1 (100% over 26bp)
TCTGATGATTCATGATAACTCGACGGATCGCATGGCCTTGGAATTCTCGG2118560.34735355322455885No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTGGAATTCTCGGGTGCCA2110490.34603041714414473No Hit
TCCGTCGTAGTCTAGCTGGTTAGGATACTCGGCTTGGAATTCTCGGGTGC1846230.3027030391255274No Hit
GACGCATTTATTAGATAAAAGGTCGACGCGGGCTTGGAATTCTCGGGTGC1668610.2735809287657801No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA1558760.2555702102486186No Hit
GAAGGTCACGGCGAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1532260.2512253396004185RNA PCR Primer, Index 1 (100% over 34bp)
GTGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACT1497760.24556880988861077RNA PCR Primer, Index 1 (100% over 25bp)
CTCTGATGATTCATGATAACTCGACGGATCGCATGGCCTGGAATTCTCGG1324790.21720910135958546No Hit
CCCTGGTTGATGAACCATGACCGTGCGGCTTGGAATTCTCGGGTGCCAAG1321020.21659098202586038No Hit
ATCCGGAGGACCGAGTGCCGCTCACGCCCGGTCGTACTCTGGAATTCTCG1317090.21594662951086316No Hit
TCTGATGATTCATGATAACTCGACGGATCGCATGGCCTGGAATTCTCGGG1282810.21032617042330468No Hit
GTTCGGATTGCAGGCTGCAACTCGCCTGCATTGGAATTCTCGGGTGCCAA1223230.20055758954708722No Hit
GACGCATTTATTAGATAAAAGGTCGACGCGGGCTGGAATTCTCGGGTGCC1167280.19138417397098173No Hit
CTCTGATGATTCATGATAACTCGACGGATCGCATGGCCTTGGAATTCTCG1166110.19119234383292913No Hit
TGGCTGTAGTTTAGTGGTTAGAATTCCACGTTGGAATTCTCGGGTGCCAA1102490.18076137512958987No Hit
CGTCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTGGAATTCTCGGGTG1075160.17628041985354045No Hit
AATCCGGAGGACCGAGTGCCGCTCACGCCCGGTCGTACTCTGGAATTCTC1051770.17244545666631783No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTGGAATTCTCGGGTGCCA1045530.1714223625967039No Hit
AACCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGG1033260.16941060550789577Illumina Small RNA Adapter 2 (100% over 21bp)
TACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCATGGAATTCTC1025700.1681710876927866No Hit
GCCCTTTTAACTCAGTGGTAGAGTAACGCCTGGAATTCTCGGGTGCCAAG969420.15894356618030733No Hit
TGCCAAGGATGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTGCCA959670.15734498169653557No Hit
CGTCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTTGGAATTCTCGGGT891020.14608930735695305No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC806170.13217752341356517No Hit
GGTAGGGTCCAGCGGCTGGAAGAGCACCGCATGGAATTCTCGGGTGCCAA794920.13033300285536703No Hit
TGGCCTGCTGGTCCGATCTCATCTGAACCACCATGGAATTCTCGGGTGCC786180.12890001532837572No Hit
TCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTTGGAATTCTCGGGTGC785010.12870818519032312No Hit
TTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAG784510.12862620649884765No Hit
TACGCTTGTGGAGACGTCGCTGCCGTGATCGTGGAATTCTCGGGTGCCAA784180.12857210056247384No Hit
AGCGGTACCAAATCGAGGCAAACTCTGAATACTAGATATGACCTCTGGAA771870.12655378517834767No Hit
TAGGATCATCGGATTCGACAACACCCGTCAAGTCTGGAATTCTCGGGTGC734610.12044473308959538No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCTGGAATTCTCGGGTGCCA720370.11810997995637389No Hit
CGCGGGGTAGAGCAGTTTGGTAGCTCGCAAGGCTGGAATTCTCGGGTGCC706410.11582113489037867No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA689040.11297319514852072No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCGGCTTGGAATTCTCGGGTGC686700.1125895348724155No Hit
CTGATTGGTCGTAGGTTCGAATCCTACTTGGGGAGCTGGAATTCTCGGGT652680.10701170470442427No Hit
CGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTTGGAATTCTCGGGTGCC617400.10122728823391484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)