FastQCFastQC Report
Mon 25 Sept 2023
SRR23951087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23951087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51829965
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA19397683.742560891175597No Hit
TCTGATGATTCATGATAACTCGACGGATCGCATGGCCTTGGAATTCTCGG5285991.0198714199401833No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTTGGAATTCTCGGGTGCC4929150.9510232160102751No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTTGGAATTCTCGGGTGCCAA4401330.8491863731723531No Hit
TCTGATGATTCATGATAACTCGACGGATCGCATGGCCTGGAATTCTCGGG4154840.8016289418678944No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCTGGAATTCTCGGGTGCCAA3754170.7243242398485124No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA3329090.6423099070200028No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC3156320.6089759080485584No Hit
CTCTGATGATTCATGATAACTCGACGGATCGCATGGCCTGGAATTCTCGG2934760.5662284356163467No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA2272000.43835646039892173No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCA2240900.43235607047004565No Hit
CTCTGATGATTCATGATAACTCGACGGATCGCATGGCCTTGGAATTCTCG2002710.38640002940384No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTGGAATTCTCGGGTGCCA1764350.34041118877853765No Hit
GAAGGTCACGGCGAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1546640.2984065298905758RNA PCR Primer, Index 3 (100% over 34bp)
CGTCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTGGAATTCTCGGGTG1398210.26976865602745437No Hit
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC1396560.26945030736563297RNA PCR Primer, Index 1 (100% over 26bp)
CGTCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTTGGAATTCTCGGGT1380480.2663478549522463No Hit
TCCGTCGTAGTCTAGCTGGTTAGGATACTCGGCTTGGAATTCTCGGGTGC1297710.25037832844378727No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA1232070.23771383986078326No Hit
ACGTCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTTGGAATTCTCGGG1025920.19793955099140043No Hit
ATCCGGAGGACCGAGTGCCGCTCACGCCCGGTCGTACTCTGGAATTCTCG1020370.196868741856183No Hit
TACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCATGGAATTCTC936280.18064453641826694No Hit
GACGCATTTATTAGATAAAAGGTCGACGCGGGCTTGGAATTCTCGGGTGC832170.16055770055025118No Hit
TGGCTGTAGTTTAGTGGTTAGAATTCCACGTTGGAATTCTCGGGTGCCAA698400.13474830631276716No Hit
GACGCATTTATTAGATAAAAGGTCGACGCGGGCTGGAATTCTCGGGTGCC680520.1312985644501207No Hit
AATCCGGAGGACCGAGTGCCGCTCACGCCCGGTCGTACTCTGGAATTCTC657750.12690535291698538No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC625620.12070623624769958No Hit
GTTCGGATTGCAGGCTGCAACTCGCCTGCATTGGAATTCTCGGGTGCCAA622760.12015443190054248No Hit
AGCGGTACCAAATCGAGGCAAACTCTGAATACTAGATATGACCTCTGGAA585950.11305236266318142No Hit
TCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTTGGAATTCTCGGGTGC581360.11216677456756916No Hit
GTGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACT556210.10731436920707935RNA PCR Primer, Index 1 (100% over 25bp)
TAGGATCATCGGATTCGACAACACCCGTCAAGTCTGGAATTCTCGGGTGC544190.10499524744035617No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA543050.10477529745582502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)