FastQCFastQC Report
Mon 25 Sept 2023
SRR23951088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23951088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75494223
Total Bases11.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA836069111.074610304950088No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCTGGAATTCTCGGGTGCCAA34193664.529308156466489No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTTGGAATTCTCGGGTGCCAA24997043.311119580633342No Hit
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC12034971.5941577410499346RNA PCR Primer, Index 1 (100% over 26bp)
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA10590491.4028212463356302No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA9425491.2485048028112031No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC5904350.782092955642447No Hit
TTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAG4870970.6452109587246166No Hit
TGCCGGCCGGGGGACGGACTGGGAATTCCCCGGGCTGGAATTCTCGGGTG4261730.5645107440870012No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCA4105730.5438469113060479No Hit
TTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAG3807640.5043617708337763No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTGGAATTCTCGGGTGCCAA3731400.49426298486441805No Hit
TGGCTGTAGTTTAGTGGTTAGAATTCCACGTTGGAATTCTCGGGTGCCAA3471320.4598126667255056No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA3454810.45762574442285475No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTGGAATTCTCGGGTGCCAA2818650.37335969402585945No Hit
TCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTTGGAATTCTCGGGTGC2668800.3535104931141552No Hit
GCCCTTTTAACTCAGTGGTAGAGTAACGCCTGGAATTCTCGGGTGCCAAG2597590.34407798329151623No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTTGGAATTCTCGGGTGCCA2420730.3206510251784431No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAGG2384850.31589834363882385Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTTGGAATTCTCGGGTGCCA2260640.2994454290893225No Hit
CTTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAA2251850.2982811015883957No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTGGAATTCTCGGGTGCC2221570.29427019866142606No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTTGGAATTCTCGGGTGCC2191850.29033347359572137No Hit
CGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTTGGAATTCTCGGGTGCC2117740.2805168284201031No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCTGGAATTCTCGGGTGCCA1885290.24972639297181717No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTGGAATTCTCGGGTGCCAAG1708220.22627161816076974No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTGGAATTCTCGGGTGCCA1698990.22504900805456335No Hit
GGTCGTAGGTTCGAATCCTACTTGGGGATGGAATTCTCGGGTGCCAAGGA1599350.21185064716806212RNA PCR Primer, Index 1 (100% over 22bp)
TCCGATGTCGTCCAGCGTTAGGATATCTGGCTGGAATTCTCGGGTGCCAA1498340.19847081544239484No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA1454510.19266507319374623No Hit
CCCTGGTTGATGAACCATGACCGTGCGGCTTGGAATTCTCGGGTGCCAAG1427780.1891244049230098No Hit
GGGTTGCTAACTCAACGGTAGAGTACTCGGCTGGAATTCTCGGGTGCCAA1307830.17323577196098833No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCTGGAATTCTCGGGTGCCAA1247060.16518614940907464No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC1230910.1630469128743798No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG1213930.16079773415245296No Hit
TCCTTTTTCCCCAGTTCAAATCCGGGTGCCGCCTGGAATTCTCGGGTGCC1148370.15211362596579078No Hit
TACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAGGA1112900.1474152532174548RNA PCR Primer, Index 1 (100% over 22bp)
TCGGGGGTCCCAGTTCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAAG1054120.13962922699396482No Hit
TGATTGGTCGTAGGTTCGAATCCTACTTGGGGATGGAATTCTCGGGTGCC1039480.13769000576375226No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGGAATTCTCGGGTGCCA1036220.13725818464281697No Hit
TAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTGGAATTCTCGGGTGC1004430.13304726641136502No Hit
TGCCGGCCGGGGGACGGACTGGGATTCCCCGGGCTGGAATTCTCGGGTGC966990.1280879465439362No Hit
TAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTGGAATTCTCGGGTG954200.12639377717683112No Hit
TTTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAA946150.1253274704211473No Hit
TGCCGGCCGGGGGACGGACTGGGAACTGGAATTCTCGGGTGCCAAGGAAC927290.12282926602211668RNA PCR Primer, Index 1 (100% over 24bp)
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC854630.11320468852298804No Hit
GGGCGCTTGAGAGAACCATGTCGAAGGAACTGGAATTCTCGGGTGCCAAG843980.11179398455428834No Hit
AGGGATATAACTCAGCGGTAGAGTGTCACCTGGAATTCTCGGGTGCCAAG842920.1116535764597511No Hit
GCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTCC835710.11069853649596474RNA PCR Primer, Index 1 (100% over 27bp)
TCCAGCGACCTGGGTTCGACTCCCGGTAGGACCTGGAATTCTCGGGTGCC832110.11022167881640428No Hit
ACCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGA817100.10823344721357024RNA PCR Primer, Index 1 (100% over 22bp)
GCACCAGTAGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG813430.10774731730135165No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA786790.10421857047260424No Hit
AACAGAAAAGATAACTCTTCCCGAGGCCCCCGCTGGAATTCTCGGGTGCC756640.10022488740628538No Hit
CTGATTGGTCGTAGGTTCGAATCCTACTTGGGGATGGAATTCTCGGGTGC756250.10017322782433301No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)