FastQCFastQC Report
Mon 25 Sept 2023
SRR23951089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23951089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63939014
Total Bases9.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA916833414.339185774744664No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCTGGAATTCTCGGGTGCCAA43802656.850692129847358No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTTGGAATTCTCGGGTGCCAA31336294.9009654731303804No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA11273831.7632161171581406No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC10351141.6189082928304148No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA9734071.5223991411566027No Hit
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC6799311.0634055132598697RNA PCR Primer, Index 1 (100% over 26bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCA5071320.793149547160674No Hit
TTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAG4899570.7662880131370183No Hit
TGGCTGTAGTTTAGTGGTTAGAATTCCACGTTGGAATTCTCGGGTGCCAA4645220.7265079189366292No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA3707730.5798853889113773No Hit
TTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAG3574020.5589732741264981No Hit
GCCCTTTTAACTCAGTGGTAGAGTAACGCCTGGAATTCTCGGGTGCCAAG3118910.4877945099372349No Hit
TGCCGGCCGGGGGACGGACTGGGAATTCCCCGGGCTGGAATTCTCGGGTG2975780.465409116255687No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTGGAATTCTCGGGTGCCAA2315870.36219982998173855No Hit
CTTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAA2220260.3472465183776528No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTGGAATTCTCGGGTGCCAA2107640.32963285921174823No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC2075870.32466406191374797No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAGG1931290.302051889633456Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGATATAGTCGAATGGTAAAATTTCTCCTTTGGAATTCTCGGGTGCCA1925700.3011776190355391No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTTGGAATTCTCGGGTGCCA1863820.2914996468353422No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTGGAATTCTCGGGTGCCAAG1796060.28090204831747956No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTATGC1763900.2758722553963688RNA PCR Primer, Index 30 (100% over 50bp)
TCCGATGTCGTCCAGCGTTAGGATATCTGGCTGGAATTCTCGGGTGCCAA1683090.26323364948980915No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG1663020.2600947208851234No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTGGAATTCTCGGGTGCC1601260.25043551656896057No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTTGGAATTCTCGGGTGCC1565080.24477699953271095No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGGAATTCTCGGGTGCCA1464130.22898851708911244No Hit
GGGTTGCTAACTCAACGGTAGAGTACTCGGCTGGAATTCTCGGGTGCCAA1433990.22427465021590728No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC1400020.21896177504395048No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCTGGAATTCTCGGGTGCCAA1318900.20627468543696967No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA1298630.20310447702556064No Hit
CCCTGGTTGATGAACCATGACCGTGCGGCTTGGAATTCTCGGGTGCCAAG1259170.19693297115904854No Hit
TCGGGGGTCCCAGTTCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAAG1189170.18598503880588463No Hit
AGGGATATAACTCAGCGGTAGAGTGTCACCTGGAATTCTCGGGTGCCAAG1188070.18581299986890634No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTGGAATTCTCGGGTGCCA1167150.18254113208564648No Hit
GCACCAGTAGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG1158980.18126335198099863No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCTGGAATTCTCGGGTGCCA1086860.1699838536765675No Hit
TACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAGGA1069050.16719838688785535RNA PCR Primer, Index 1 (100% over 22bp)
TTTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAA1038330.16239380857515257No Hit
GGGCGCTTGAGAGAACCATGTCGAAGGAACTGGAATTCTCGGGTGCCAAG1006590.15742970324816083No Hit
CGCGGGGTAGAGCAGTTTGGTAGCTCGCAAGGCTGGAATTCTCGGGTGCC927260.1450225679113538No Hit
GGTCGTAGGTTCGAATCCTACTTGGGGATGGAATTCTCGGGTGCCAAGGA913450.14286269725710815RNA PCR Primer, Index 1 (100% over 22bp)
GGGTCCATAGCTCAGTGGTAGAGCATTTGATGGAATTCTCGGGTGCCAAG832710.13023503928290167No Hit
TGATTGGTCGTAGGTTCGAATCCTACTTGGGGATGGAATTCTCGGGTGCC829110.12967200276188182No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTTGGAATTCTCGGGTGCCAAGG754370.1179827389893751Illumina Small RNA Adapter 2 (100% over 21bp)
TGGCTGTAGTTTAGTGGTTAGAATCCCACGTTGGAATTCTCGGGTGCCAA736020.11511281672250999No Hit
TGCCGGCCGGGGGACGGACTGGGAACTGGAATTCTCGGGTGCCAAGGAAC726550.11363171787416053RNA PCR Primer, Index 1 (100% over 24bp)
TGGCTGTAGTTTAGTGGTTAGAATCCCACGTGGAATTCTCGGGTGCCAAG695320.10874737605431327No Hit
TCCTTTTTCCCCAGTTCAAATCCGGGTGCCGCCTGGAATTCTCGGGTGCC665210.10403820115211661No Hit
CTGATTGGTCGTAGGTTCGAATCCTACTTGGGGATGGAATTCTCGGGTGC659150.10309042300839985No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA658410.10297468772352354No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC653380.10218800058443192No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)