FastQCFastQC Report
Mon 25 Sept 2023
SRR23951090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23951090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59739942
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA694080411.618364142368936No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTTGGAATTCTCGGGTGCCAA22429713.754558382396823No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCTGGAATTCTCGGGTGCCAA21742413.6395097270097785No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA8611141.441437623089758No Hit
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC8594161.4385953036244996RNA PCR Primer, Index 1 (100% over 26bp)
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA7053511.1807025189277887No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC6862611.1487473489679652No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCA4536710.7594098434176585No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTGGAATTCTCGGGTGCCAA3711290.6212409781047327No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA3117550.5218535364497007No Hit
TGGCTGTAGTTTAGTGGTTAGAATTCCACGTTGGAATTCTCGGGTGCCAA3049520.5104658454472554No Hit
TTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAG2478610.41489996759621894No Hit
TGCCGGCCGGGGGACGGACTGGGAATTCCCCGGGCTGGAATTCTCGGGTG2429680.40670946751170267No Hit
TTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAG2385650.3993391891810006No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTGGAATTCTCGGGTGCCAA2229500.37320089798547174No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTTGGAATTCTCGGGTGCCA2190330.3666441457207977No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTGGAATTCTCGGGTGCC1654590.27696545135581147No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTTGGAATTCTCGGGTGCCA1629740.2728057553186108No Hit
TAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTGGAATTCTCGGGTGC1555950.2604538852749472No Hit
GCCCTTTTAACTCAGTGGTAGAGTAACGCCTGGAATTCTCGGGTGCCAAG1545830.25875987626502883No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTGGAATTCTCGGGTGCCAAG1504480.25183820901600473No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCTGGAATTCTCGGGTGCCA1496570.25051413675627604No Hit
CCCTGGTTGATGAACCATGACCGTGCGGCTTGGAATTCTCGGGTGCCAAG1489120.24926706490608913No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC1452780.2431840325522914No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGC1419200.23756300265574412RNA PCR Primer, Index 29 (100% over 50bp)
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTTGGAATTCTCGGGTGCC1334470.22337986200254428No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA1289700.2158857134477968No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAGG1279330.21414985638921444Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCAGTGGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG1238140.2072549718913353No Hit
CTTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAA1164960.19500521108641183No Hit
GGGTTGCTAACTCAACGGTAGAGTACTCGGCTGGAATTCTCGGGTGCCAA1124430.18822080543700562No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGGAATTCTCGGGTGCCA1078210.18048393820000697No Hit
TCCGATGTCGTCCAGCGTTAGGATATCTGGCTGGAATTCTCGGGTGCCAA1052330.17615182820231062No Hit
GGGCGCTTGAGAGAACCATGTCGAAGGAACTGGAATTCTCGGGTGCCAAG1017270.1702830578576725No Hit
TCCAGCGACCTGGGTTCGACTCCCGGTAGGACCTGGAATTCTCGGGTGCC1007690.16867944063286838No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCTGGAATTCTCGGGTGCCAA988810.16551907599776378No Hit
TGCCGGCCGGGGGACGGACTGGGAACTGGAATTCTCGGGTGCCAAGGAAC954530.15978087156495732RNA PCR Primer, Index 1 (100% over 24bp)
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTGGAATTCTCGGGTGCCA946900.15850366911973232No Hit
GCACCAGTAGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG929660.1556178276838635No Hit
TAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTGGAATTCTCGGGTG918370.15372796980619766No Hit
AGGGATATAACTCAGCGGTAGAGTGTCACCTGGAATTCTCGGGTGCCAAG886290.14835802820163435No Hit
ATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTGGAATTCTCGGGTG856790.1434199584592834No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC837840.1402478763705529No Hit
TCCAGTAACCCGAGTTCAATTCTCGGTAGAACCTGGAATTCTCGGGTGCC776500.12998003915035605No Hit
TACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAGGA759230.12708917594864755RNA PCR Primer, Index 1 (100% over 22bp)
TCCAGCGACCTGGGTTCGACTCCCGGTAGGACCTTGGAATTCTCGGGTGC753350.12610490984407047No Hit
TGCCGGCCGGGGGACGGACTGGGAATGGAATTCTCGGGTGCCAAGGAACT700740.11729840648322024RNA PCR Primer, Index 1 (100% over 25bp)
GCACCAGTGGTCTAGTGGTAGAATAGTACTGGAATTCTCGGGTGCCAAGG699940.11716449272749545Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGATATAGTCGAATGGTAAAATTTCTCCTGGAATTCTCGGGTGCCAAG687110.11501685087005943No Hit
AGGGATATAACTCAGCGGTAGAGTTGGAATTCTCGGGTGCCAAGGAACTC665600.11141624476300965RNA PCR Primer, Index 1 (100% over 26bp)
AGAGCCCCGTCGTGCCCGGACCCTGTCGCATGGAATTCTCGGGTGCCAAG655720.10976240987980873No Hit
GGTCGTAGGTTCGAATCCTACTTGGGGATGGAATTCTCGGGTGCCAAGGA655360.10970214868973258RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTTATCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA640910.1072833314769539No Hit
TAGGACTCCGCTGGCACCTTATGAGAAATCAAATGGAATTCTCGGGTGCC631080.10563786620348577No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGTGGAATTCTCGGGTGCCAAG622120.10413803213936833No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTTGGAATTCTCGGGTGCCAAGG618610.1035504855361259Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGCCCCGTCGTGCCCGGACCCTGTCGCACTGGAATTCTCGGGTGCCAA616010.10311526583002038No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)