FastQCFastQC Report
Mon 25 Sept 2023
SRR23951091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23951091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62762384
Total Bases9.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA1002544015.973644340852317No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTTGGAATTCTCGGGTGCCAA40959706.526154264630865No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCTGGAATTCTCGGGTGCCAA39627656.313917266112773No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC17755012.828925363956857No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA11084301.7660737680072827No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA9563791.5238092294263392No Hit
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC6925941.1035176738984294RNA PCR Primer, Index 1 (100% over 26bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCA6285371.001454947919123No Hit
TGGCTGTAGTTTAGTGGTTAGAATTCCACGTTGGAATTCTCGGGTGCCAA5101570.8128387857287256No Hit
GCCCTTTTAACTCAGTGGTAGAGTAACGCCTGGAATTCTCGGGTGCCAAG3837030.6113582301143946No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA3816540.6080935357713627No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGGAATTCTCGGGTGCCA3584310.5710920732392829No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC3340210.5321993504899367No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTTGGAATTCTCGGGTGCCA3330730.5306888916138048No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTGGAATTCTCGGGTGCCAA2891280.4606708374876264No Hit
TTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAG2603400.4148025989580001No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTGGAATTCTCGGGTGCC2591690.41293683171754597No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC2118180.3374919601524378No Hit
TTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAG2087380.3325845621160598No Hit
TGCCGGCCGGGGGACGGACTGGGAATTCCCCGGGCTGGAATTCTCGGGTG2045080.32584485637129396No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTGGAATTCTCGGGTGCCAAG2002060.3189904322308726No Hit
GGGTTGCTAACTCAACGGTAGAGTACTCGGCTGGAATTCTCGGGTGCCAA1896090.30210611502584095No Hit
TCCGATGTCGTCCAGCGTTAGGATATCTGGCTGGAATTCTCGGGTGCCAA1847360.29434191027542866No Hit
CGCGGGGTAGAGCAGTTTGGTAGCTCGCAAGGCTGGAATTCTCGGGTGCC1761460.2806553683492966No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTTGGAATTCTCGGGTGCC1720600.2741450993958419No Hit
CCCTGGTTGATGAACCATGACCGTGCGGCTTGGAATTCTCGGGTGCCAAG1699530.2707879930118652No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTGGAATTCTCGGGTGCCAA1693280.26979217360513263No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG1662730.26492460834502396No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTTGGAATTCTCGGGTGCCA1590480.25341293600319575No Hit
TGCCGGCCGGGGGACGGACTGGGAACTGGAATTCTCGGGTGCCAAGGAAC1571170.2503362523641549RNA PCR Primer, Index 1 (100% over 24bp)
CTTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAA1524850.24295603557697873No Hit
TCGGGGGTCCCAGTTCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAAG1438740.22923603411878044No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCTGGAATTCTCGGGTGCCA1394530.22219200596331712No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCTGGAATTCTCGGGTGCCAA1297690.20676238174764044No Hit
AGGGATATAACTCAGCGGTAGAGTGTCACCTGGAATTCTCGGGTGCCAAG1290480.20561360448003377No Hit
GTGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACT1264530.20147896230328027RNA PCR Primer, Index 1 (100% over 25bp)
GCACCAGTGGTCTAGTGGTAGAATAGTACCCTGGAATTCTCGGGTGCCAA1175190.18724432137568262No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTGCTGGAATTCTCGGGTGC1172240.18677429461570486No Hit
GCACCAGTAGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG1120410.1785161634395532No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA1074220.17115665969603702No Hit
GGGTCCATAGCTCAGTGGTAGAGCATTTGATGGAATTCTCGGGTGCCAAG1044270.1663846930989747No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC1015620.16181985693851272No Hit
GGGCGCTTGAGAGAACCATGTCGAAGGAACTGGAATTCTCGGGTGCCAAG992710.1581695813211939No Hit
GCACCAGTAGTCTAGTGGTAGAATAGTACCCTGGAATTCTCGGGTGCCAA991510.15797838399510128No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAGG890080.1418174300071202Illumina Small RNA Adapter 2 (100% over 21bp)
TGGCTGTAGTTTAGTGGTTAGAATCCCACGTTGGAATTCTCGGGTGCCAA849130.13529282125420858No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTTTGGAATTCTCGGGTGCCA836230.1332374499987126No Hit
TTTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAA792450.1262619342184325No Hit
TGGCCTGCTGGTCCGATCTCATCTGAACCACCTGGAATTCTCGGGTGCCA769230.12256226595853975No Hit
GTGCCGGCCGGGGGACGGACTGGGAACGGCTCTGGAATTCTCGGGTGCCA764080.12174171076739214No Hit
AGAAGCGTCCTCAGCGACGGACCGGGCCTGGAATTCTCGGGTGCCAAGGA758700.12088450942207678RNA PCR Primer, Index 1 (100% over 22bp)
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTTGGAATTCTCGGGTGC751910.11980265121860253No Hit
TGCCGGCCGGGGGACGGACTGGGAATGGAATTCTCGGGTGCCAAGGAACT744060.11855190204374645RNA PCR Primer, Index 1 (100% over 25bp)
GGGCGCTTGAGAGAACCATGTCGAAGGAACTTGGAATTCTCGGGTGCCAA732970.11678492008844023No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTGGAATTCTCGGGTGCCA726830.1158066271032662No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACTGGAATTCTCGGGTGCCAAGG725680.11562339633242741Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGAACCCTGAACAGACTGCCGGTTGGAATTCTCGGGTGCCAAGGAACT724490.11543379231738551RNA PCR Primer, Index 1 (100% over 25bp)
TCCGTCGTAGTCTAGCTGGTTAGGATACTCGGCTTGGAATTCTCGGGTGC675430.10761700830229776No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGATGGAATTCTCGGGTGCCA674390.10745130395301747No Hit
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC671370.10697012401568429RNA PCR Primer, Index 2 (100% over 34bp)
TGGCTGTAGTTTAGTGGTTAGAATCCCACGTGGAATTCTCGGGTGCCAAG629670.10032601693396477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)