FastQCFastQC Report
Wed 27 Sept 2023
SRR23951092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23951092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80579990
Total Bases12 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA60347897.489190554627768No Hit
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC39118824.854656844707972RNA PCR Primer, Index 1 (100% over 26bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCTGGAATTCTCGGGTGCCAA26153483.245654411225417No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTTGGAATTCTCGGGTGCCAA25637293.181595083345133No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC8899581.1044404448300378No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA7365710.9140867354289819No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA7099700.8810748177059837No Hit
TTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAG4779140.5930926524066334No Hit
TTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAG4527490.5618628148253679No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCA4458950.5533569810569597No Hit
TTGGAACCCTGAACAGACTGCCGGTTGGAATTCTCGGGTGCCAAGGAACT3996770.4960003097543199RNA PCR Primer, Index 1 (100% over 25bp)
GTGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACT3636270.45126215577837625RNA PCR Primer, Index 1 (100% over 25bp)
TGGCTGTAGTTTAGTGGTTAGAATTCCACGTTGGAATTCTCGGGTGCCAA3355070.416365154674256No Hit
TGGAACCCTGAACAGACTGCCGGTTGGAATTCTCGGGTGCCAAGGAACTC3338490.4143075718922278RNA PCR Primer, Index 1 (100% over 26bp)
GCCCTTTTAACTCAGTGGTAGAGTAACGCCTGGAATTCTCGGGTGCCAAG3205540.39780843854659204No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTGGAATTCTCGGGTGCCAA3091980.3837156097934487No Hit
TGCCGGCCGGGGGACGGACTGGGAACTGGAATTCTCGGGTGCCAAGGAAC3070600.3810623456269975RNA PCR Primer, Index 1 (100% over 24bp)
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC3033290.37643216386599204RNA PCR Primer, Index 2 (100% over 34bp)
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTTGGAATTCTCGGGTGCCA2981100.369955369813275No Hit
CTTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAA2798120.3472474990379125No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA2710590.3363850007923803No Hit
AGCGTCCTCAGCGACGGACCGGGCCTGGAATTCTCGGGTGCCAAGGAACT2645990.3283681221603527RNA PCR Primer, Index 1 (100% over 25bp)
TGCCGGCCGGGGGACGGACTGGGAATGGAATTCTCGGGTGCCAAGGAACT2598620.3224894914978272RNA PCR Primer, Index 1 (100% over 25bp)
CCCTGGTTGATGAACCATGACCGTGCGGCTTGGAATTCTCGGGTGCCAAG2445480.3034847733289617No Hit
TGGTCAGGATACTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2166620.2688781668997477RNA PCR Primer, Index 1 (100% over 33bp)
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTGGAATTCTCGGGTGCC2154450.26736786638965826No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC1999180.24809881460645503No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAGG1975000.24509806963242364Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCCGGACCCTGTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1969480.24441303604033704RNA PCR Primer, Index 1 (100% over 33bp)
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGGAATTCTCGGGTGCCA1897010.23541948813843241No Hit
AAGCGTCCTCAGCGACGGACCGGGCCTGGAATTCTCGGGTGCCAAGGAAC1693530.21016756145042959RNA PCR Primer, Index 1 (100% over 24bp)
TGCCGGCCGGGGGACGGACTGGGAATTCCCCGGGCTGGAATTCTCGGGTG1603790.1990308015674859No Hit
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1601890.1987950110194851RNA PCR Primer, Index 1 (100% over 29bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCTGGAATTCTCGGGTGCCA1599240.19846614525516817No Hit
GGGTTGCTAACTCAACGGTAGAGTACTCGGCTGGAATTCTCGGGTGCCAA1524290.18916482863797823No Hit
GCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTCC1490790.18500746897585865RNA PCR Primer, Index 1 (100% over 27bp)
AGAAGCGTCCTCAGCGACGGACCGGGCCTGGAATTCTCGGGTGCCAAGGA1484040.18416979202901365RNA PCR Primer, Index 1 (100% over 22bp)
GCACCAGTGGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG1466200.18195584288357444No Hit
TACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAGGA1452350.18023705388893696RNA PCR Primer, Index 1 (100% over 22bp)
TTTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAA1408700.17482007629933932No Hit
TTTGGAACCCTGAACAGACTGCCGGTTGGAATTCTCGGGTGCCAAGGAAC1369660.16997520103936475RNA PCR Primer, Index 1 (100% over 24bp)
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTGGAATTCTCGGGTGCCAAG1339380.1662174443059623No Hit
GTGCCCGGACCCTGTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1251670.15533260800851428RNA PCR Primer, Index 1 (100% over 32bp)
AGGGATATAACTCAGCGGTAGAGTTGGAATTCTCGGGTGCCAAGGAACTC1245990.15462771837028028RNA PCR Primer, Index 1 (100% over 26bp)
TCCGATGTCGTCCAGCGTTAGGATATCTGGCTGGAATTCTCGGGTGCCAA1235910.15337678746299174No Hit
AGAGCCCCGTCGTGCCCGGACCCTGTCGCATGGAATTCTCGGGTGCCAAG1196950.14854184022609088No Hit
GAGAAGCGTCCTCAGCGACGGACCGGGCCTGGAATTCTCGGGTGCCAAGG1185830.14716184501884402Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGGGGTCCCAGTTCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAAG1184260.1469670075660223No Hit
TTGGAACCCTGAACAGACTGCCGGTGTGGAATTCTCGGGTGCCAAGGAAC1124240.13951850825496503RNA PCR Primer, Index 1 (100% over 24bp)
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC1075940.13352446432420756No Hit
GCACCAGTAGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG1067370.13246092485243546No Hit
AGCCCCGTCGTGCCCGGACCCTGTCGCTGGAATTCTCGGGTGCCAAGGAA1049370.1302271196608488RNA PCR Primer, Index 1 (100% over 23bp)
AGCCCCGTCGTGCCCGGACCCTGTCGCATGGAATTCTCGGGTGCCAAGGA1029590.1277724159558719RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTCGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1008660.12517499691921036RNA PCR Primer, Index 2 (100% over 34bp)
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCTGGAATTCTCGGGTGCCAA989470.12279351238440213No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTGGAATTCTCGGGTGCCAA961290.11929636625668481No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTTGGAATTCTCGGGTGCCA940190.11667785017099158No Hit
AGAGCCCCGTCGTGCCCGGACCCTGTCGCACTGGAATTCTCGGGTGCCAA921670.11437951282942575No Hit
ACCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGA868740.10781088456327681RNA PCR Primer, Index 1 (100% over 22bp)
GAGCCCCGTCGTGCCCGGACCCTGTCGCATGGAATTCTCGGGTGCCAAGG829530.10294491225427058Illumina Small RNA Adapter 2 (100% over 21bp)
TGATCCGGGAGTCCCGTGTGGAAGGGCTCTTGGAATTCTCGGGTGCCAAG822180.1020327751343727No Hit
AGGGATATAACTCAGCGGTAGAGTGTCACCTGGAATTCTCGGGTGCCAAG817700.10147680584224444No Hit
GGGTCCATAGCTCAGTGGTAGAGCATTTGATGGAATTCTCGGGTGCCAAG806760.10011914868691346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)