FastQCFastQC Report
Mon 25 Sept 2023
SRR23951093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23951093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66412194
Total Bases9.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA842203612.68146027520187No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCTGGAATTCTCGGGTGCCAA38196515.751430226804432No Hit
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTTGGAATTCTCGGGTGCCAA34791925.2387849135054925No Hit
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC12729441.9167323398471068RNA PCR Primer, Index 1 (100% over 26bp)
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC12679841.909263831880031No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA10103581.5213441073788347No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA9278851.3971605877077333No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCA6258280.9423389927458201No Hit
TGGCTGTAGTTTAGTGGTTAGAATTCCACGTTGGAATTCTCGGGTGCCAA4552620.6855096520376966No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTGGAATTCTCGGGTGCCAA3661600.551344531698501No Hit
TTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAG3531580.5317668017412586No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA3482100.5243163627450705No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTTGGAATTCTCGGGTGCCA3435240.5172604296132725No Hit
GCCCTTTTAACTCAGTGGTAGAGTAACGCCTGGAATTCTCGGGTGCCAAG3174880.47805678577641936No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTGGAATTCTCGGGTGCC3000960.45186882396928485No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGGAATTCTCGGGTGCCA2679940.4035313153485036No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTGGAATTCTCGGGTGCCAA2635010.396765991498489No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC2583190.3889632075699833No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTTGGAATTCTCGGGTGCCA2479060.3732838580818456No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCTGGAATTCTCGGGTGCCA2148840.32356106169297766No Hit
TTTACTAAAGAATACCGTGTGCTCTCCACGTGGAATTCTCGGGTGCCAAG2125510.3200481526028187No Hit
CTTCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAA1954000.29422307596102004No Hit
TGCCGGCCGGGGGACGGACTGGGAACTGGAATTCTCGGGTGCCAAGGAAC1900660.2861914184012653RNA PCR Primer, Index 1 (100% over 24bp)
TGGCTGTAGTTTAGTGGTAAGAATTCCACGTGGAATTCTCGGGTGCCAAG1838090.2767699558307018No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTCGTATGC1804050.2716443910887811RNA PCR Primer, Index 28 (100% over 50bp)
TGCCGGCCGGGGGACGGACTGGGAATTCCCCGGGCTGGAATTCTCGGGTG1730670.26059521539071573No Hit
GGGTTGCTAACTCAACGGTAGAGTACTCGGCTGGAATTCTCGGGTGCCAA1724850.2597188703026435No Hit
CCCTGGTTGATGAACCATGACCGTGCGGCTTGGAATTCTCGGGTGCCAAG1650600.24853869456563957No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC1616150.24335139417318452No Hit
TCCGATGTCGTCCAGCGTTAGGATATCTGGCTGGAATTCTCGGGTGCCAA1534070.23099221808573286No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGGAATTCTCGGGTGCCAAGG1421260.21400587970335688Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCAGTGGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG1394720.21000962564194162No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTTGGAATTCTCGGGTGCC1387600.20893753336924842No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCTGGAATTCTCGGGTGCCAA1326420.19972536971147198No Hit
TGCCGGCCGGGGGACGGACTGGGAATGGAATTCTCGGGTGCCAAGGAACT1298830.19557101215478592RNA PCR Primer, Index 1 (100% over 25bp)
GCGGATATAGTCGAATGGTAAAATTTCTCCTTGCTGGAATTCTCGGGTGC1114690.1678441763270161No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA1084410.16328477267292207No Hit
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1026720.15459811491847417RNA PCR Primer, Index 2 (100% over 34bp)
AGGGATATAACTCAGCGGTAGAGTGTCACCTGGAATTCTCGGGTGCCAAG978700.14736751506809126No Hit
TCGGGGGTCCCAGTTCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAAG881070.1326669015030583No Hit
GCACCAGTAGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG875930.13189294725001857No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC849010.12783947478079102No Hit
TGGCTGTAGTTTAGTGGTTAGAATCCCACGTTGGAATTCTCGGGTGCCAA828290.12471956580744795No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTTGGAATTCTCGGGTGC784250.11808825349151994No Hit
TTGGAACCCTGAACAGACTGCCGGTTGGAATTCTCGGGTGCCAAGGAACT768550.11572422980032854RNA PCR Primer, Index 1 (100% over 25bp)
TGGAACCCTGAACAGACTGCCGGTTGGAATTCTCGGGTGCCAAGGAACTC761840.11471387317816967RNA PCR Primer, Index 1 (100% over 26bp)
TGGTCAGGATACTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC760220.1144699420711805RNA PCR Primer, Index 1 (100% over 33bp)
GGGTCCATAGCTCAGTGGTAGAGCATTTGATGGAATTCTCGGGTGCCAAG747920.11261787255515154No Hit
AGGGATATAACTCAGCGGTAGAGTTGGAATTCTCGGGTGCCAAGGAACTC742340.11177766540885548RNA PCR Primer, Index 1 (100% over 26bp)
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTGGAATTCTCGGGTGCCA692260.10423688155822709No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCCTGGAATTCTCGGGTGCCAA689110.10376257107241481No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)