FastQCFastQC Report
Mon 25 Sept 2023
SRR24044788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24044788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64804547
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGA3006390.4639165211663311Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGA2623500.4048327041002231Illumina DpnII expression Sequencing Primer (96% over 27bp)
GGCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGAT1542920.2380882316791752Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG938130.14476299016487224No Hit
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAG839440.1295341204992915No Hit
GGGCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAGAT813420.1255189701426352Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG757960.11696092868298269No Hit
GGGCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGA715060.11034102282977148No Hit
GGGTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGAT708320.10930097235306653Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAGAT677100.10448340916571795Illumina DpnII expression Sequencing Primer (96% over 27bp)
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAAAAAAAAAAAAAAA658730.10164873153113779No Hit
CGCCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGA650980.1004528277930868Illumina DpnII expression Sequencing Primer (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)