FastQCFastQC Report
Mon 25 Sept 2023
SRR24044789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24044789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78943082
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGA1615880.20468924686776224Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAG1426940.18075554739552732No Hit
GGCCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGA1360900.17239002652569355Illumina DpnII expression Sequencing Primer (96% over 27bp)
GGGCTCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAGAT1334580.169055978838019No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1280610.16221940764866513No Hit
GGCCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAAAAAAAAAAAAAAAG1178510.14928603876904628No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1060620.13435249462391144No Hit
GGCCTCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAGAT1006710.12752352384721943No Hit
GGGCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGA979650.12409573773671516No Hit
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCAAAAAAAAAAAAA973150.1232723596983457No Hit
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAAAAAAAAAAAAAAA959340.12152299805067149No Hit
GGCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGAT834550.10571540644942136Illumina DpnII expression Sequencing Primer (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)