FastQCFastQC Report
Mon 25 Sept 2023
SRR24044790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24044790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72106228
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGA2466550.3420717001033531Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGA2084620.28910401470452735Illumina DpnII expression Sequencing Primer (96% over 27bp)
GGCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGAT1336560.18535985546213846Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1135560.15748431605658253No Hit
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAG990210.13732655659092305No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG924840.12826076549171314No Hit
GGCCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAAAAAAAAAAAAAAAG759870.10538202053780985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)