FastQCFastQC Report
Mon 25 Sept 2023
SRR24044791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24044791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72134877
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGA2296850.3184104687667243Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGA2009540.2785809144722046Illumina DpnII expression Sequencing Primer (96% over 27bp)
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1206570.1672658289831145No Hit
GGGCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGA1197820.16605282351836545No Hit
GGCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGAT1186060.16442254417374275Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAG1060120.14696358323311481No Hit
GGCCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAAAAAAAAAAAAAAAG997250.13824796568239797No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG975530.13523693954590094No Hit
GGCCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGA885650.12277694741199877No Hit
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAAAAAAAAAAAAAAA860240.11925437954236756No Hit
GGCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT855960.1186610465836103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)