FastQCFastQC Report
Mon 25 Sept 2023
SRR24044794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24044794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60680309
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGA1947260.3209047600598079Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGA1613100.2658358249296324Illumina DpnII expression Sequencing Primer (96% over 27bp)
GGGCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGA1264200.2083377657157283No Hit
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAG1092560.18005181878688192No Hit
GGCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGAT974460.1605891624579565Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG935210.15412083679402488No Hit
GGCCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGA900470.1483957505885476No Hit
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAAAAAAAAAAAAAAA855140.14092545243960442No Hit
GGCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT813190.13401217188923675No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG782700.12898747763463103No Hit
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCAAAAAAAAAAAAAA706590.11644469378031677No Hit
GGGCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCAAAAAAAAAAAAAAA644000.10612998032030457No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)