FastQCFastQC Report
Mon 25 Sept 2023
SRR24044801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24044801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64948737
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCAAAAAAAAAAAAA1125280.1732566408489206No Hit
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAG1036540.15959355760836427No Hit
GGGCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGA1020420.15711160018400358No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG918130.14136225620522844No Hit
GGGCTGTTCAGAGTTCTACAGTCCGACGATCAGAAAAAAAAAAAAAAAGA780340.12014706305990214Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAAAAAAAAAAAAAAA763060.11748650324024006No Hit
GGCCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGA751240.11566660641915177No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG748550.11525243362315114No Hit
GGCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT684440.10538157193110621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)