FastQCFastQC Report
Fri 29 Sept 2023
SRR24204965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24204965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58665486
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC22777273.8825673412131967No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT20706593.5296034196324566No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT13770202.347240420031635No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT10783351.8381080146510675No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6596571.1244379702232417No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG6239681.063603223196685No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA5946841.0136863095278883No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG5871231.000797981968478No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT5381730.9173588027549964No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC5262960.8971135089548223No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC5068990.8640497753653655No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG5024720.8565036007713291No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC3706210.6317530549393215No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA3443250.5869294255910537No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT3416450.5823611518363625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC3293750.5614459581908177No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC3237360.551833832928615No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG2965980.5055749474230896No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG2929760.4994009595352197No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2897880.4939667592628484Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT2442730.41638281152226364Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2362960.4027853787830207No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA2265770.3862185681032285No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2163920.368857423255643No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2016580.34374214508339707No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT1977710.33711644355933573No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA1975320.33670904899688375No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG1914300.32630770330616543No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1900340.3239281099623039Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1829830.3119091180800923No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT1766900.3011821976553642No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1739420.29649801247704655Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1724260.2939138695620795Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT1708070.29115415493191343Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACT1702450.2901961811072357Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA1686300.28744328479610654No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1663450.2835483200463046No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC1642980.28005904527919534Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC1637910.2791948233412743No Hit
AGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGA1536080.2618370876532072Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC1504250.2564114102796319No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1461030.24904421656031284Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1457910.24851238767543832No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC1380860.23537860063070132No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1342030.22875971742567686No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACA1283350.2187572433986143No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1227830.20929341657546313No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1224850.20878545180721764Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA1188310.20255691736705295Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1184600.20192451827638486No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT1170550.19952958371469046No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT1110970.18937369750929872No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1110040.1892151715916919No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1082470.18451564519554137No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1068260.18209343735769956Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1049710.17893144190435922No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1037900.17691833320872855Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC1034500.1763387760905961No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1027840.17520352597096014Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC987530.16833236496157214No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG916250.15618212043790108No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGGAGATCGGAAGAGCACACGTCTG908100.1547928879341424Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGAGGTAGTGACGAAAAATAACAAAGATCGGAAGAGCACACGTCT906770.15456617882616705Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAGATCGGAAGAGCACA904580.15419287585889938No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT893640.15232806560232023No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGTAGATCGGAAGAGCACACG885990.15102406208652222No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC854190.14560349845222453No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCTG842350.14358527601731622Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCCGCTAAGGAGTGTGTAACAACTCACCTGCAGATCGGAAGAGCACAC837520.1427619639936163No Hit
CCGGGGAGGTAGTGACGAAAAATAACAAAGATCGGAAGAGCACACGTCTG820890.1399272478540449Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC784730.1337634874447303No Hit
TAAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCT771040.13142991775436755Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC740560.126234358648286No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG724330.12346782569908311TruSeq Adapter, Index 3 (100% over 49bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG722380.12313543264603655No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT717380.12228314276643No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG697950.11897114429427892No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC687660.11721713172204863No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA673060.11472844527359749Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA660550.1125960159948219Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT657360.11205225505163292No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT633080.10791353539626347Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTG632080.10774307742034217Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGAGATCGGAAGAGCAC629500.1073032958424652No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACAC627430.10695044783230807No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG622300.10607599841583176Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACT596080.10160659028717499Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)