FastQCFastQC Report
Fri 29 Sept 2023
SRR24204966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24204966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63023985
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA14422042.2883414941152957No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACAC10196161.6178221672272866No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC9715431.541544857882281No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC8745121.3875860118968992No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG5526660.8769137654497728No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC4770720.7569689539625272No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4653290.7383363651155984Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGA4537290.7199306740124415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT4347860.6898738631014844Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC4282610.6795206618559585Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA3644660.5782972942761395No Hit
GCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACT3359310.5330208808598822Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC3161050.5015630160485727No Hit
ACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCT2968620.47103019588494766Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG2648350.4202130347676365No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2449380.38864251443319553No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGTAGATCGGAAGAGCACACG2224390.35294340718061545No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC2103070.33369359300272744No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1993350.3162843479351552No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1893300.30040943935868225No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1890990.300042912234128No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT1853570.29410549015585097Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACG1823780.2893787182768592No Hit
ACGTGTTAGGACCCGAAAGATGGAGATCGGAAGAGCACACGTCTGAACTC1816210.28817758826262735Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC1749210.2775467149530452Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA1636690.25969319458298296No Hit
AGTCGGGTTCAGATCCCCGAATCCGGAGAGATCGGAAGAGCACACGTCTG1625540.2579240268605675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCCGCTAAGGAGTGTGTAACAACTCACCTGCAGATCGGAAGAGCACAC1618080.25674035051893335No Hit
GCACGAGCGCACGTGTTAGGACCCGAAAGATGGAGATCGGAAGAGCACAC1569410.24901789374315192No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAGATCGGAAGAGCACA1504240.23867738607769723No Hit
AGCACGAGCGCACGTGTTAGGACCCGAAAGATGGAGATCGGAAGAGCACA1492300.23678286925207917No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC1478270.23455673264710253No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1450750.2301901411026294No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGAGATCGGAAGAGCAC1422300.22567598668982927No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA1421070.22548082289623544No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC1407800.22337527530193463No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC1387250.22011461192115986No Hit
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA1379100.21882145345141218Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG1365580.21667623841938907No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1318580.20921876012759266No Hit
AGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGA1313510.208414304490584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACACGTGCGCGAGTCGGGGGCTAGATCGGAAGAGCACACGTCTGAACT1288640.2044681877859675Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAGGCGAGCGAGAAAACCAATGTTAAGGAAAGATCGGAAGAGCACACGT1243740.19734391597103865No Hit
CTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGAA1212110.19232519175041057Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTC1152240.18282563376466912No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC1147840.18212748686075628No Hit
ATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAACT1134600.18002669935898213Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1133080.17978552133763043No Hit
TAAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCT1111900.17642489601379538Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAGGCGAGCGAGAAAACCAATGTTAAGGAACAGATCGGAAGAGCACACG1111320.1763328675582796No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGGAGATCGGAAGAGCACACGTCTG1079610.17130144975758038Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACGAGCGCACGTGTTAGGACCCGAAAGATGGAGATCGGAAGAGCACACG1064560.16891347000669665No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCAGATCGGAAGAGCACACGTC1035150.16424699263304282No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA1017520.16144964492486472No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTAGATCGGAAGAGCACACG1000200.1587014848394623No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGAGATCGGAAGAGCACA974080.15455703094623421No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTG968620.1536906941063787Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACACGT967960.15358597207079178No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACAC960670.15242926958680889No Hit
ACGTGTTAGGACCCGAAAGATGGTAGATCGGAAGAGCACACGTCTGAACT949480.1506537550743578Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGTCTGA931460.14779452616333288Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA930470.14763744310995253No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC929570.14749464033415213No Hit
TCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACG927270.1471296999071068No Hit
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACA902490.14319786348007033No Hit
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT883070.14011649691780043Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAA872160.1383854099355983Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG854400.13556743515980463Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA833690.13228138461888755Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA802590.12734675536623083No Hit
CTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTG799740.12689454657619637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCT788640.1251333123413253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAGGCGAGCGAGAAAACCAATGTTAAGGAAGATCGGAAGAGCACACGTC787830.12500478984310495No Hit
TCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGAACT785280.12460018197833729Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGAACTC781710.12403373096766254Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCTGAAC780670.12386871442673769Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGAAC775600.12306425878972901Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT746440.11843744885379748No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACA746080.11838032774347734No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC740150.11743941612070388No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA729280.11571467592853737Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG725880.1151751987755138TruSeq Adapter, Index 2 (100% over 49bp)
AAGGCGAGCGAGAAAACCAATGTTAAGGAACAGATCGGAAGAGCACACGT725630.11513553133779147No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT722860.11469601612782816Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGAGATCGGAAGAGCACAC721110.11441834406377191No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG715120.1134679122559451Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGA715070.11345997876840064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT713270.1131743732167999No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAAAGATCGGAAGAGCACACGTC702890.11152738120256914No Hit
GACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC696080.11044684019901313No Hit
CGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGT692930.10994703048371188No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT689190.10935360561538596No Hit
CACGTGTTAGGACCCGAAAGATGGAGATCGGAAGAGCACACGTCTGAACT686620.10894582435560049Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAAGATCGGAAGAGCAC681590.10814771550862738No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC673540.10687042401396865No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT669610.10624685189297377Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAGGCGAGCGAGAAAACCAATGTTAAGGAACTAGATCGGAAGAGCACAC666040.10568040088229902No Hit
ACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGT658990.10456177913852956No Hit
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCA658270.10444753691788927No Hit
GCACGAGCGCACGTGTTAGGACCCGAAAGATGGTAGATCGGAAGAGCACA643110.10204210349440772No Hit
GATTCAACCCGGCGGCGGGTCCGGCCGTGTCGAGATCGGAAGAGCACACG639970.10154388047661538No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCAC632870.10041732524530146No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)