FastQCFastQC Report
Fri 29 Sept 2023
SRR24204967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24204967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39321327
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC7774691.9772196396118575No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC7762981.974241611937461No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT7381551.8772382732658033No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA6643521.6895462353038084No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5897721.4998781704391613No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3611670.9185015551484312No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC3303430.8401115252290443No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG3183710.8096649434033596No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG2673750.6799745084900111No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2420370.6155361949000347No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2288870.5820937833557855No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC2174560.5530230452293738No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2130720.5418738792818462No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG2126870.540894766852604No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC2087050.5307679468701553Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT2073970.5274415077598983No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1942050.49389228395064083No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1675170.42602071898539945No Hit
GCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACT1648500.41923814015737565Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG1622700.412676815306869No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT1550630.3943483392612869Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1548590.3938295368312468Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1541690.3920747639061113Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA1328350.3378192195802548No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1283460.3264030229702065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCCGCTAAGGAGTGTGTAACAACTCACCTGCAGATCGGAAGAGCACAC1256740.3196077283963484No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT1246230.31693487862197534No Hit
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA1241220.3156607608893769Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCAGATCGGAAGAGCAC1190080.3026550960500392No Hit
AGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGA1176770.2992701645089445Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA1113050.2830652180176931No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1064360.27068262472423676No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC995040.25305351469954207Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG951450.24196792748118595No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA914980.23269306246963636No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC901040.22914791253102929Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA878680.22346143099392346Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC870300.22133027199209224Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA850870.2163889331608773No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA817240.20783632251271683No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG798040.20295347611233974No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT780460.19848261987699448No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC768090.19533674435758488No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG695920.17698283682033417No Hit
AGAATCCCGCCCAGGCGGAACGATACAGATCGGAAGAGCACACGTCTGAA694430.17660390759447156Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT673470.1712734669407266Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGAGATCGGAAGAGCACAC646220.16434338546102475No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC641710.16319642518676952No Hit
TAAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCT620460.1577922332071855Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC619950.1576625325996755No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCTG615040.15641384635874572Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA610450.15524654089115558Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC603980.1536011233801952No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAGATCGGAAGAGCACA602760.1532908591818379No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT597740.15201419830007262No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT592010.15055697382746008Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG585670.1489446172556689No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGGAGATCGGAAGAGCACACGTCTG574550.1461166353821172Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGTAGATCGGAAGAGCACACG573870.1459437012387705No Hit
ATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGAGATCGGAAGAGCACA548130.139397635283265No Hit
CTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACT523580.13315420407861617Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTG507030.12894529220745782Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACA488470.12422520735375996No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACACGT481330.12240939884861973No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT481280.12239668310278541No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC480190.12211947984359735No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACA479430.12192620050691576No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT473930.12052746846514108No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC463280.11781901460243191No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC458300.1165525263173341No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG455800.11591673902561833TruSeq Adapter, Index 1 (100% over 49bp)
CCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTG453610.11535978935807534Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAA449450.11430183930466029Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC430640.10951817572179087No Hit
CTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTG428830.10905786572258866Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC414750.10547711169564547No Hit
AATCCGCTAAGGAGTGTGTAACAACTCACCTGAGATCGGAAGAGCACACG410910.10450054241557005No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACAGATCGGAAGAGCA410700.10444713628306593No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACAC409790.10421570970888139No Hit
CAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAAC399550.10161152496201362Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGT397440.1010749204878055No Hit
CTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGA393630.10010598065523069Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)