FastQCFastQC Report
Fri 29 Sept 2023
SRR24204968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24204968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18935579
Total Bases965.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC3574221.8875683706318143No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC3528161.8632437909609205No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT3382531.78633565944828No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3017721.5936771724804404No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2690931.4210972899217922No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1627200.8593346947563631No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC1511450.798206381753629No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1475300.7791153362672459No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1229000.649042735899441No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1086630.5738562311720175No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1047300.5530858074104837No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC990350.5230101493067627Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC986200.5208185078470534No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC983460.5193714963772694No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG963440.5087988067330818No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT950810.5021288232063038No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC866640.45767810955239346No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC779890.4118648814488324No Hit
GCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACT772170.4077879002273973Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA754040.3982133316335349Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG737390.3894203604759062No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT728320.38463043564709587Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT723100.3818737203652447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG609440.32184914968800266Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA588200.31063217026529794Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA582590.30766949349687167No Hit
AATCCGCTAAGGAGTGTGTAACAACTCACCTGCAGATCGGAAGAGCACAC581290.30698295520828806No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT577390.3049233403425372No Hit
AGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGA554440.29280329901715707Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCAGATCGGAAGAGCAC541220.2858217327286374No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA506450.2674594740409047No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT490050.25879852947723436No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC480090.25353858997393214Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG433050.22869646605472166No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC430800.22750822670909612Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA422490.2231196627259193Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA417090.2202678882964181No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC409740.21638630643404144Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA375290.19819304178657543No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA371490.1961862375584079No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG362870.19163396059872265No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC354750.18734573682695416No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT352480.186146935353812No Hit
AGAATCCCGCCCAGGCGGAACGATACAGATCGGAAGAGCACACGTCTGAA323760.17097972023987223Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG314910.1663059788137453No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT312510.16503852351174475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCT295230.15591284533734087Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA294090.15531080406889064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCTG293590.15504675088097386Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGAGATCGGAAGAGCACAC287910.15204710666623925No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC284440.15021457754209683No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC282060.14895768436761295No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC278550.14710403098843716No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT274570.1450021676126196Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT271470.1433650378475356No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAGATCGGAAGAGCACA265010.1399534706596508No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGGAGATCGGAAGAGCACACGTCTG262050.13839027578718346Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG260810.13773542388114987No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGTAGATCGGAAGAGCACACG258420.13647324964290766No Hit
CTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACT253660.13395946329393993Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGAGATCGGAAGAGCACA244630.1291906627201629No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTG239550.12650788233092847Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT219820.11608834353573239No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACA218270.11526977865319037No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT217650.11494235270017358No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC216630.11440368419682334No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACACGT216140.1141449120726649No Hit
CAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAA214090.1130622940022061Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC213380.11268733847536429No Hit
CCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTG212560.11225429124718078Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACA211550.1117209038075889No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC210310.11106605190155526No Hit
CTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTG200130.10568992899556967Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC197020.10404751816672729No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC192970.1019086873446014No Hit
AATCCGCTAAGGAGTGTGTAACAACTCACCTGAGATCGGAAGAGCACACG192430.10162350990165128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)