FastQCFastQC Report
Fri 29 Sept 2023
SRR24204969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24204969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20022546
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC3823701.9096971983483018No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC3768271.8820134062870926No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT3615851.8058892210810755No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3250471.62340493561608No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2871811.4342881269944392No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1723870.8609644347926583No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC1613790.8059864115182955No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1577430.7878268827550703No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1310510.6545171628023729No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1166260.5824733777612497No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1124410.561571939952092No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1067210.5330041444279864No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1062650.5307267117778128No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC1061400.5301024155469539Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1039920.5193745091158737No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1017410.5081321825905656No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC929620.46428660970487967No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC839160.419107540070079No Hit
GCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACT836070.41756427978739563Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA806250.40267106890402454Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG797150.3981261923433713No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT780460.3897905890689426Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT778470.38879670946941514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG649610.32443925962262743Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA633490.31638833542947037Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA623180.3112391401173457No Hit
AATCCGCTAAGGAGTGTGTAACAACTCACCTGCAGATCGGAAGAGCACAC622660.3109794328853084No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT614500.30690402709026116No Hit
AGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGA593900.2966156252057056Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCAGATCGGAAGAGCAC583970.2916562159477621No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA546420.2729023571727592No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT526710.2630584542045752No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC521880.2606461735685362Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG467070.23327203243783284No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC464690.2320833724142774Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA451700.22559568598319113Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA444430.22196477910451545No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC439420.2194625998112328Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA403930.2017375812246854No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA396560.198056730647541No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG390690.19512503554742738No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC379050.1893115890456688No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT378030.1888021633212879No Hit
AGAATCCCGCCCAGGCGGAACGATACAGATCGGAAGAGCACACGTCTGAA349570.17458818673709128Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG336430.16802558475830198No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT334790.16720650810341503Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA319270.15945524610107026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCT318110.15887589919883316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCTG315060.15735261639553733Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGAGATCGGAAGAGCACAC312050.15584931107162894No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC308580.1541162647347645No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC305370.15251307201391873No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC295980.14782335872770624No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT295410.14753867964643458Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT291370.14552095422829844No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG285620.14264919156634726No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAGATCGGAAGAGCACA283480.1415803964191167No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGTAGATCGGAAGAGCACACG278470.13907821712583404No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGGAGATCGGAAGAGCACACGTCTG278030.1388584648525717Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACT276310.13799943323890979Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGAGATCGGAAGAGCACA263160.1314318368902736No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTG256060.12788583429899472Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACA233810.11677336138970539No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC233310.11652364289736179No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT232320.11602920028252152No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT231330.11553475766768122No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACACGT231060.1153999096818157No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACA231030.11538492657227506No Hit
CAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAA229890.11481556840973171Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTG228460.11410137352162907Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC227710.11372679578311369No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC225520.11263302878664881No Hit
CTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTG213520.10663978497040287Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC210980.10537121502929747No Hit
AATCCGCTAAGGAGTGTGTAACAACTCACCTGAGATCGGAAGAGCACACG206730.10324860784437703No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC206500.10313373733789899No Hit
CAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAAC203440.10160546016475627Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)