FastQCFastQC Report
Fri 29 Sept 2023
SRR24204970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24204970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18282533
Total Bases932.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC3485321.90636603801016No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC3456721.8907226914344963No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT3310011.810476699262624No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2966191.6224174188554725No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2624461.4355013060826964No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1583860.8663241575989498No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC1476720.8077217746578113No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1433170.7839012241903242No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1185100.6482143366020453No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1057610.5784811109051465No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1020330.5580900633407855No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC971480.5313705710253607Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC964800.5277168103566393No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC958180.5240958678975173No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG937950.5130306615609554No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT923490.5051214730475257No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC837650.45816955451413655No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC765910.4189299152365813No Hit
GCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACT759370.4153527303902583Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG730420.3995179442585992No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA721520.394649909834704Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT715480.39134621006837506Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT702380.3841809009725293Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG583790.3193157096995258Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA573050.31344125018125224Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA566920.3100883231005244No Hit
AATCCGCTAAGGAGTGTGTAACAACTCACCTGCAGATCGGAAGAGCACAC566470.3098421865285297No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT563540.3082395639597642No Hit
AGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGA539440.295057583104048Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCAGATCGGAAGAGCAC528100.2888549414897815No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA497220.27196450294934515No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT483970.2647171483295012No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC468160.2560695501000873Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG421970.23080499841023122No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC415130.22706372251591178Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA407610.22295050691280033Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA403610.22076262627284743No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC399420.21847082130249676Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA366190.20029500288608806No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA360820.19735777312695127No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG353090.1931296937902423No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC347620.1901377670151067No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT342210.1871786584495704No Hit
AGAATCCCGCCCAGGCGGAACGATACAGATCGGAAGAGCACACGTCTGAA317960.17391463206985597Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT303740.1661367163948234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG302050.1652123368244433No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCTG287620.15731955741581322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCT287220.15710076935181794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA284970.15587008649184442Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGAGATCGGAAGAGCACAC283010.1547980249782675No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC281440.153939281827086No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC278890.15254450791911603No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC270180.14778039782561858No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT265690.14532450180727144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT265560.14525339568647297No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAGATCGGAAGAGCACA260800.14264981772492905No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG256310.1401939217065819No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGGAGATCGGAAGAGCACACGTCTG255800.13991496692498792Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGTAGATCGGAAGAGCACACG253850.13884837511301087No Hit
CTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACT247250.13523837205708858Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGAGATCGGAAGAGCACA240280.13142599004197067No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTG230630.1261477279980843Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACA214400.11727040230147541No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT212800.11639525004549425No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC212650.11631320452149602No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACACGT211200.11552009778951308No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT211010.11541617345911533No Hit
CAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAA209360.11451367269513475Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC208370.1139721722367464No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACA208320.113944823728747No Hit
CCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTG205270.11227656474078292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC202420.11071769978481646No Hit
CTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTG195250.10679592373770089Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC192780.10544490744252998No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC186930.10224513200659886No Hit
AATCCGCTAAGGAGTGTGTAACAACTCACCTGAGATCGGAAGAGCACACG186180.1018349043866077No Hit
CAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAAC183570.10040731226903844Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)