FastQCFastQC Report
Mon 25 Sept 2023
SRR24207626.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24207626.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35492983
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA694886319.57813182397208Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA33554469.453829225906428Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC24840896.9988172028256965Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC20108925.66560438157593Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC13821613.8941809991005827Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC9372592.6406881608119557Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8741772.4629572555228734Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6096191.7175761192008008Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT5965661.6807998358436087Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5864841.6523942211337943Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5203721.4661264171568786Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA4678471.318139419276199Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4141681.1669010745025292Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT3739591.0536138932024959Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT3623201.020821495899626Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3435060.967813835202299Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2863690.8068327195829104Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2306080.6497284266019568Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2287470.6444851366818055Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA2235590.6298681629549142Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2003040.5643481698903696Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1932690.5445273506597065Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1801110.5074552341796686Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCAC1792310.5049758708643903Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1786100.5032262292521313Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT1687440.475429185537885Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1605100.4522302337901551Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1522610.4289890201677329Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTC1439150.4054745130889674Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1372340.3866510741010413Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1312380.3697575940573944Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1232900.3473644353871299No Hit
GGCAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC1227180.3457528492321989TruSeq Adapter, Index 5 (97% over 35bp)
GGGAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCAC985650.2777027786027452Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT891500.2511764085875791Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA871430.24552176975375667Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC844250.2378639180595218TruSeq Adapter, Index 5 (97% over 35bp)
CGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA813510.22920305120592427Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC749330.2111206037542688TruSeq Adapter, Index 5 (97% over 35bp)
GGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC717350.2021103720698821Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT693590.1954160911186304Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT679640.19148573677225156Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTC670350.18886831799964518Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA653180.18403074207653947Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA634830.17886070607252144No Hit
GCGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT579430.16325198701951876Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCAC570890.16084587762037358Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC531540.14975917915944117Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG523420.1474714030094343Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520480.14664307026546627No Hit
CGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT487660.13739617208280297Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA466990.13157248575021152Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCACT446760.125872767583384Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC443540.12496554600665716Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA442440.12465562559224735Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA440860.12421046717882235No Hit
GACAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC424940.1197250735448187Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC412770.1162962267781212Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA412120.11611309198778812Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA405040.11411833150231412Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGCAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA397140.1118925394351892Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC388210.1093765491618442TruSeq Adapter, Index 5 (97% over 35bp)
GACAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC383650.10809178817119992Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)