FastQCFastQC Report
Mon 25 Sept 2023
SRR24207627.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24207627.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36326261
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA746894720.56073703814439Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA366703710.094727337889248Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC26880707.3997981790639Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC21320425.869147942310936Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC15494954.265495422168552Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC9950292.7391451049696527Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC9886382.7215517721463267Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6522331.7954861911056577Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT6497701.788705972244157Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5996021.6506020259007663Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5766651.5874603774938465Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4325141.1906372637690403Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT4277431.1775035146061412Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3822021.0521369099891673Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT3807451.0481260375242032Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT3320040.9139503787631764Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT3297350.9077042088091588Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2625940.7228764887198272Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2525720.6952876322724213Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2124050.5847147329586163Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2088210.574848592317277Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1897530.5223576409364014Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1812520.49895583803684057Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1798970.4952257541727182Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT1764100.4856266379851205Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1604310.44163917668267594Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1433680.39466764828893347Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC1348430.37119977748329236TruSeq Adapter, Index 5 (97% over 35bp)
GCCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1342700.36962240622562287Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1328060.3655922639547186No Hit
GGGAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC982800.27054807539922704TruSeq Adapter, Index 5 (97% over 35bp)
GGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA947390.2608003064229484Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA916400.25226928805031706Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC876490.241282745835031TruSeq Adapter, Index 5 (97% over 35bp)
CGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT823290.22663769332054295Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC780320.2148087853027318Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT758860.2089012133673763Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA728020.20041148743604525No Hit
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA695820.19154737670359193Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT639510.1760461942394787Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA544170.14980071854904087No Hit
GGCAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG543450.14960251483079967Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA531570.14633215347982Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC516430.14216436973791494Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT514010.14149818501827094Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA508920.1400969948434825No Hit
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA493570.1358714016837571Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC485320.13360031741224346Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA473960.13047310319110464Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GACAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC459660.12653655712048095Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC448390.1234341183641223TruSeq Adapter, Index 5 (97% over 35bp)
GCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC441250.12146859815823048Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC403190.11099132938564749Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA386790.10647668913682035No Hit
TCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA367950.10129035850950914Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA363640.100103889029482Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)