FastQCFastQC Report
Mon 25 Sept 2023
SRR24207628.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24207628.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34544702
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA718308720.79359955109759Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA348184410.07924167358572Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC25376657.346032395937299Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC19648285.68778390388199Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC14319684.145260827550343Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC9397952.720518474873513Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC9112892.6379993088375753Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6186891.7909808572093053Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA6086251.7618475909851532Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5704551.6513530786862771Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5511851.5955702845547777Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4271631.2365514109804738Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3901631.12944381456815Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3503491.0141902512286833Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT3442020.9963959162247225Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3015990.873068755955689Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2410760.6978667814242543Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2325860.6732899302474805Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA2180960.6313442796524921Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1966180.5691697673350895Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1943640.5626448883536468Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1710140.4950513106177613Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1670100.48346053180600607Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1658280.4800388783206177Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1607140.46523487161649274Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT1582780.4581831390526976Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1394980.4038187968736856Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1358880.3933685692237264No Hit
GCCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1280050.3705488615880953Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG1232410.3567580348500329TruSeq Adapter, Index 13 (100% over 35bp)
GGGAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA1053130.30486006218840733Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1030150.2982078120112311Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG868860.25151758437516697TruSeq Adapter, Index 13 (100% over 35bp)
GGCAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCAC822450.23808281802517794Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA811690.23496801332951145Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG774550.22421672648963656TruSeq Adapter, Index 13 (100% over 35bp)
GAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA742600.2149678407994372Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA729980.21131460332180604Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC693330.20070516167717992Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA662030.1916444379806779No Hit
GGCAAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTC651150.18849489568617497Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA635130.18385742624151163Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA593690.17186137544333138Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC531230.15378045524896988Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA522900.15136908692974105No Hit
GAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA498520.14431156476613982Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG494260.14307838000744658Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA475400.1376187873903211Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCAC462290.13382370471744118Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA455800.13194497958037096Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC450140.1303065228352527Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC443150.1282830577030307Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA439070.1271019793425921Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA420400.12169738792362429No Hit
GCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG397610.11510013894460575TruSeq Adapter, Index 13 (100% over 35bp)
GCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC394680.11425196257301626Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC378630.10960580872864382Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT357990.10363094172877799Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)