FastQCFastQC Report
Mon 25 Sept 2023
SRR24207629.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24207629.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55087454
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA715717612.992388430222244Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA34123386.194401360425915Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC23688274.300120677205376Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC18133693.2918003435047116Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC13149322.386989966898815Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8611811.563297879041569Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC8214001.4910836140657362Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5961681.0822210080720014Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5709361.0364174753837778Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT5663731.0281342826263127Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5061230.9187627367930273Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA4432040.80454616762648No Hit
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4324840.7850862012973044Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTG3755800.6817886337604203Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT3565770.6472925759102971Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3291630.5975280687323106Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA3172260.5758588879420712No Hit
GGCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2946360.5348513656122136Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2783770.5053364782478421Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA2211460.4014453091261034Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2196500.3987296272577781Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC2162650.39258485244208235No Hit
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2026260.367826038938013Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAGTACCGTAAGGGAAAGTTGAAAAAAAAAAAAAAAGATCGGAAGAG1913800.34741122724604406No Hit
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1912940.34725511184452273Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1811440.328829863874268Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC1779550.3230408869504116No Hit
GGGAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCT1724760.3130948836372071Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1707030.30987636495235377Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA1663880.30204336544578736No Hit
GGGATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACA1648780.29930226944233074No Hit
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1587960.28826164302311014Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1533150.2783120091191726Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC1488000.27011595053930065Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1445460.26239368405009245Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC1433400.2602044378380602Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1343610.24390490074200924Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA1311360.23805057318495784No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1308500.237531398710131No Hit
GGGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT1297690.2355690644189147Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGTCAGCGGAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA1280330.23241771166262284Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1221370.2217147301815764Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1198120.2174941684543998Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA1186530.21539024112459437TruSeq Adapter, Index 10 (100% over 35bp)
GGGGTCAGCGGAGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCT1097090.19915423936637186Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA988420.1794274246183169Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA879240.15960802980656905Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT875850.1589926446773162Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA825430.14983992543928423TruSeq Adapter, Index 10 (100% over 35bp)
GGGATTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG824360.14964568883506577No Hit
GGGCAGCGGAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC791630.1437042271004211Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGTAGTCAGCGGAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCT772530.14023701295035343Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA746290.13547367790858514Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA743710.13500533170402101TruSeq Adapter, Index 10 (100% over 35bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA722600.13117324318528137No Hit
GGCTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC720210.13073938759268128No Hit
GAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT694670.12610312322656989Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA687920.12487779885416378Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCT672320.12204593808238079Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAG665340.12077886191654455No Hit
CGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT664590.12064271476405498Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCAGCGGAGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA641030.11636587888051607Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGATATTAGTCAGCGGAGGAAAAAAAAAAAAAAGATCGGAAGAGCACAC618910.1123504455297571No Hit
GGGAGTCAGCGGAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTG594660.10794835426592778Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTAGTCAGCGGAGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT592350.10752902103625991No Hit
GGCAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCT588130.10676296639158527Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC586320.10643439793024378Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA576830.10471168262740913Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAAGTACCGTAAGGGAAAGTTGAAAAAAAAAAAAAAAAGATCGGAAGA574420.104274196444076No Hit
GGCAAGTACCGTAAGGGAAAGTTGAAAAAAAAAAAAAAAGATCGGAAGAG558230.10133523324566789No Hit
GGGAAGTACCGTAAGGGAAAGTTGAAAAAAAAAAAAAAGATCGGAAGAGC557990.10129166615687121No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAA555030.10075433872837906No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)