FastQCFastQC Report
Mon 25 Sept 2023
SRR24207631.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24207631.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31143902
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA546703917.554123436427457Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA26766628.594497889185497Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC19004066.102016375468944Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC15240744.893651412080606Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC10925383.5080318452068084Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC7062032.2675482346431735Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6888212.2117363456897596Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4684661.5041981573150338Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4664881.497846994252679Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT4552941.4619041634538923Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4021201.291167689906037Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA3456681.1099058814146026No Hit
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3274981.0515638021208775Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2963940.9516919235104195Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2626940.8434845447433016Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2620120.8412947099563824Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA2539920.8155432803506767No Hit
GGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2223980.7140980600311418Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1779400.5713478034961708Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1772940.5692735611613471Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA1645250.5282735605833848No Hit
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1579640.5072068361889914Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1454100.46689717942215464Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1374420.4413127166916978Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1302730.4182937642174703Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA1292880.4151310262920812No Hit
GCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1210090.3885479732115777Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT1199150.3850352470284552Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1179070.3785877569226875Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1065280.3420509093561879Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC998720.3206791493243204Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA913810.2934153851370326TruSeq Adapter, Index 10 (100% over 35bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA872290.28008372232869216No Hit
GGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA782120.2511310239802321Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAG694390.22296178558486343No Hit
GGGGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTG661420.21237544351378965Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA659280.2116883106041112TruSeq Adapter, Index 10 (100% over 35bp)
GGGCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA600680.19287242812413163Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA598440.19215318619998226Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT573900.18427363404880995Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA573270.18407134725764293TruSeq Adapter, Index 10 (100% over 35bp)
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAA569280.18279019758025183No Hit
CGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT536290.17219743370628382Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC527280.1693044115024508Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA514370.1651591377342505Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC480080.15414895667216008No Hit
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAGA472130.15159629002171918No Hit
GGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC456860.14669324351200438Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT454710.14600289970087885Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA437980.14063106157988808No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAG424510.13630597733065047No Hit
GGGGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAGATC398170.12784846291900095No Hit
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA385920.12391510864630899Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT384490.12345594973937435Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA384380.1234206298234563No Hit
GAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA382010.12265964618049466Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT370680.11902169484093547Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG370550.11897995312212324Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA354420.11380076908795822No Hit
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC349740.11229806721071753No Hit
CCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC348650.11194807895298411Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA345510.11093985589859615Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GACAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC324260.10411669032351822Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)