FastQCFastQC Report
Mon 25 Sept 2023
SRR24207632.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24207632.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29106643
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA392765613.494019217537383Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA16750945.755023002824475Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC13105444.502559776474394Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC10512023.6115535549736877Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6612632.2718628183950997Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA4701971.6154284779594816No Hit
GGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4584901.5752074191448324Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC4221281.4502806111993058Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4007751.3769193513659408Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA3646921.2529510874888594No Hit
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3027231.0400477993975465Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG2988321.0266797170666504Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2966451.0191659684010967Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA2778120.954462525960139No Hit
GCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2630630.9037902447217977Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA2429300.8346204679117409No Hit
GGCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT1779990.6115408087425266Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1730920.5946821143200884Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1668300.5731681252283198Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1369290.4704389990972164Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1297180.4456645859160055Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1201310.41272708776481026Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1143510.39286907803143084Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA1127230.38727585314458973Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1084680.37265719719034585Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1037850.35656808653612165Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAG986610.33896385783822613No Hit
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC932200.3202705306826349Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA929010.31917456094129437Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC883860.30366263811323074Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAA832990.2861855281627634No Hit
GCGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC803320.2759919788757501Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA746560.25649127589189863Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG741690.25481811832439766Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT716120.24603318218456177Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAGA694650.23865685919190338No Hit
GGGGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTG680560.23381603986416433Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAG670380.23031855648897745No Hit
GGCAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA602980.2071623306061094TruSeq Adapter, Index 9 (100% over 35bp)
GGGCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA581000.19961078988050943Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA560320.19250588259181933Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAGATC558200.19177752652547392No Hit
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA550280.18905649820214582No Hit
GGGTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC529140.18179355138962608No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA521500.17916872103732473No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAG503010.17281621930773675No Hit
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC487790.16758717245406832No Hit
GGGAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA469860.16142706666653384Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGCGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA446960.1535594468932745No Hit
GGCGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA417420.14341056094995222No Hit
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA412000.1415484430822201No Hit
GGCAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCAC405330.13925686998668999Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC401030.13777954400306486Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA392850.13496918899235477TruSeq Adapter, Index 9 (100% over 35bp)
CGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA392090.13470808021385358Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAA380880.13085672573096113No Hit
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA374120.12853423185902957Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC367440.12623922312167707Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTTGGTGGTAGTAGCAA356530.12249093789345614No Hit
GCGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA351840.12087962187875806No Hit
GGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA342400.11763637599842758TruSeq Adapter, Index 9 (100% over 35bp)
GAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG341730.11740618799632786Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG336460.11559560475593149Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA332990.11440343704356423No Hit
GGCAAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTC332440.11421447674333311Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC326450.11215652729172512Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA321620.11049711229151365No Hit
GGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC314750.10813682635953588Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC308350.10593801559321012Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACA301920.10372889790141722No Hit
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAGA301190.10347809604838319No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA301050.10342999706286982No Hit
CGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG299800.1030005418350718Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC294120.1010490972799577Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT293950.10099069136897718Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)