FastQCFastQC Report
Mon 25 Sept 2023
SRR24207633.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24207633.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31593033
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA444572614.071855652478824Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA21422266.780691173272284Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC14851564.700897188313639Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC12012873.802379467650352Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8388202.655079048599101Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5877621.860416503853872Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA5473151.7323914421258635No Hit
GGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC5469211.731144331726555Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA4160011.3167491706161927No Hit
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3897621.2336960493789881Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3789801.1995682719034921Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG3413421.0804344109664936Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3385551.0716128457815368Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2861880.9058579465922123Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA2444280.7736769052847823No Hit
GGGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG2212040.7001670273316272Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2158020.6830683207908529Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2061440.6524982897336891Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA1958360.6198708430431482No Hit
GGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1781660.5639407903634956Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1531510.48476194102668146Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1372900.43455783431745854Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1255870.3975148571522082Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1195060.3782669425882599Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1121390.3549485103250454Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAG1084500.3432718852919249No Hit
GGGGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTG1021990.323485877408478Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1020640.32305856800769966Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG970580.3072133023758751Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA962670.3047095858127961Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT952190.3013923987608281Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT932360.29511569845161745Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC888960.28137849253029934Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAA866650.27431680902558486No Hit
GCCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC845860.26773624425359854Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAGA786900.2490739018314576No Hit
GGGCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA770080.24374994322324167Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC736110.23299757259773063No Hit
GGCAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA690550.21857667163516717TruSeq Adapter, Index 9 (100% over 35bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA686260.21721877731713823No Hit
GGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA673130.2130627977377164Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA635300.20108863875146146No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAG633310.20045875304216595No Hit
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC625240.19790439240195773No Hit
GGCAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA574500.1818438894423337Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAGATC550740.17432324398863508No Hit
CGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA510150.16147547467189996Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA492490.15588563465875532TruSeq Adapter, Index 9 (100% over 35bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA487510.15430933775810635Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA487320.1542491979165153No Hit
GAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG482110.15260010015499303Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA447390.1416103354179385TruSeq Adapter, Index 9 (100% over 35bp)
CGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG434760.13761261857954568Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC417380.13211140570137725Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAA414640.1312441258805383No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAG407430.12896197715489996No Hit
GGCGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA400450.12675262929013495No Hit
GGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC389260.12321070914590568Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA387350.12260614547517484No Hit
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA380910.12056772137072119Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA367680.11638008924309355Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA365650.11573754251451578No Hit
GGCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC351550.11127453321749765Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAG346940.10981535074521018No Hit
GCGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG341220.10800482498783831Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA338000.10698561293561147No Hit
GGGGTCAGCGGAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA335260.10611833311477249Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAGA329850.10440593025683859No Hit
GAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA328890.10410206579406288Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGTCAGCGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA327980.10381402760539007Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)