FastQCFastQC Report
Mon 25 Sept 2023
SRR24207635.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24207635.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31660021
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA462586314.6110547431412Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA22250987.028100202460384Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC15338934.84488939536711Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC12476073.9406385738025884Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8656542.734218022154818Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA6152541.9433151986854338No Hit
GGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6068301.9167075094485884Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC5682611.7948851013080505Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA5051591.5955737995246435No Hit
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3943661.2456277271578564Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3840091.2129145460768962Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3528431.114474939861853Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3480001.0991780453967481Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2795990.8831295468818546Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA2630100.8307322348270079No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA2296010.7252079839113184No Hit
GGGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2280540.7203216953014655Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2205820.6967209529014526Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2151840.6796710589673962Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1823100.575836636368624Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1562900.49365096757200505Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1384680.437359153994244Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1250380.39493972540321437Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1232220.3892037847985003Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAG1178320.3721791593252575No Hit
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1131410.3573623656156134Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1033230.3263516470819776Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA1001500.31632954381173656Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA993530.3138121734031699Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT983420.31061887166783625Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA963420.30430175646440666Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAA938750.2965095948609762No Hit
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAGA894110.2824097937269214No Hit
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC862530.27243506882070606Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC856610.2705652027204909No Hit
GCCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC815580.25760564088065513Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAG753570.23801942519242172No Hit
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA710740.22449132298427724No Hit
GGCAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT701540.22158544999069962TruSeq Adapter, Index 1 (100% over 35bp)
GGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA699850.22105165375600985Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA685830.2166233559984057No Hit
GGGGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTG616100.1945987338416484Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA595320.18803525114528508No Hit
GGGGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAGATC561470.17734353366348052No Hit
CGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA524460.16565371197953405Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC511050.16141808623563453No Hit
GGGAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT503630.15907443649516215TruSeq Adapter, Index 1 (100% over 35bp)
GAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA485110.15322478781678636Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA476930.15064108769858364Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAG476260.15042946433926876No Hit
GGGCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA470730.14868278198552048Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAA458000.14466193815853753No Hit
GGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT446440.14101064557095525TruSeq Adapter, Index 1 (100% over 35bp)
CGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA441620.13948822080692871Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA441480.1394440010005047Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA434130.13712246116324434No Hit
GGCGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA433420.1368982035735226No Hit
GGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC428560.13536314457908918Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA384610.12148128391955267No Hit
TCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA376090.11879019284289168Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAGA367290.11601066215338265No Hit
GGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC363680.1148704228591636Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA358870.1133511566527388No Hit
GGCAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCAC358600.11326587559749249Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA353830.11175924362147455Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA344370.10877124813025235No Hit
GCGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA341690.10792475469299277Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA326330.10307321021675886No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)