FastQCFastQC Report
Mon 25 Sept 2023
SRR24207636.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24207636.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33315051
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA574345517.239820524362997Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA27677708.30786661560266Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC19490465.8503467396763105Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC15672654.7043752086707Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC11087153.3279702918659795Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7370952.2124984890462875Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC7207132.1633255191474867Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4682111.4054038218341613Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA4636781.3917973590975443No Hit
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC4536911.36181991737008Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4524871.3582059352092843Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4221191.2670519399775195Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA3719811.1165553971386686No Hit
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3256200.9773960724238423Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2942180.8831383749044839Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2714550.8148118998827287Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2683090.8053687205821777Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2280380.6844894219132367Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1893120.5682476667978086Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1716450.5152175813868632Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA1557280.4674403770235861No Hit
GCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1489340.4470471919733816Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1475280.44282687725736936Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1321390.3966345421473315Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA1311810.3937589649795223No Hit
GCGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1294930.3886921860032572Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1254040.3764184542295913Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1230230.3692715343584496Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT1230040.36921450307850345Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA1049930.3151518513359022Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAG1038310.31166393832025047No Hit
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1019810.30611089264128694Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC963370.28916960085097876Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA909510.2730027338094125No Hit
GGCAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT909080.27287366301795546TruSeq Adapter, Index 12 (100% over 35bp)
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC892020.2677528544080572No Hit
GGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA877250.2633194228038252Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAGA739700.22203177776915303No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAA720540.2162806234335346No Hit
GGGAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT656140.19695002117811555TruSeq Adapter, Index 12 (100% over 35bp)
CGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA631280.18948792844411375Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAGATC613720.1842170375185678No Hit
GAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC585180.1756503389413992Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT579320.17389137420200856TruSeq Adapter, Index 12 (100% over 35bp)
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAG563320.16908874010128336No Hit
CGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC555740.16681349219606478Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC530080.1591112677570267Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA522190.15674296881610655Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA506960.15217146148147873Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA500230.15015135351286119No Hit
GGGGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTG472640.14186981133542312Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440890.1323395842917965No Hit
GGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC432300.12976117010896968Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC426570.12804122677164745Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAA399770.11999681465293269No Hit
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA397350.11927041624519799No Hit
GAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA391130.11740339223854107Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCAC383740.11518517561326862Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GGCAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG379410.11388546275975983Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA366490.11000733572342422Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA353490.10610519551658498No Hit
CCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC352720.10587406875048758Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC347850.10441226699607935Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)