FastQCFastQC Report
Mon 25 Sept 2023
SRR24207637.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24207637.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30213167
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA345372411.431188263051006Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA16674285.518878573702651Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC11425003.7814638895684123Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC9707673.2130593922841655Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA7104362.3514118860826474No Hit
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6459142.1378559884172357Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA5475231.8121999590443465No Hit
GGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC4445411.4713485679935505Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4366421.4452043375658037Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA3351041.1091323196935958No Hit
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3003690.9941658880050542Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2814750.9316302392264936Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA2735670.905456220461761No Hit
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2704550.895156075495164Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2445780.8095079870309524Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2059290.6815869385688696Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC1948760.6450035509352594No Hit
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAG1868280.6183661580396388No Hit
GGGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1751310.5796512494039436Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT1685320.5578097787630142Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1682140.5567572575228542Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1376450.4555795160434522Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAGA1339490.44334643898800813No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAA1308010.4329271406734686No Hit
GGGGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTG1178090.38992602132705917Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1134480.37549191714989694Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAGATC1009490.3341225367072575No Hit
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1008050.3336459233154869Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAG996940.3299687186053683No Hit
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC945620.3129827468931013Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA925940.3064690305389038Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA911160.3015771236428144Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC893700.2957981862675965Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC852460.2821485082977233No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT805820.26671153010871057Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC794120.2628390463005748Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA794040.2628125677788098No Hit
GGGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT781070.2585197374376542Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA778460.25765587566507014Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAA739240.24467478036976398No Hit
GCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT731020.24195411225840707Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCT705490.2335041540001417Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA671240.22216803686948808Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC637750.21108346569560218Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC633440.20965693533551116Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA629170.20824364423630268No Hit
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAGA591550.1957921193762971No Hit
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCCC588310.19471973924481337Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAG576690.1908737339584427No Hit
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCC563890.1866371704760378Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGCAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC559560.18520402048550552TruSeq Adapter, Index 5 (97% over 35bp)
GGCGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA548060.1813977329817824No Hit
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT544890.18034852155684308No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA538630.17827657722872944No Hit
GGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA503670.16670546321741114Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA496760.16441838089995664No Hit
GGCGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA472360.15634243176162232No Hit
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAAG425490.14082932782253513No Hit
GGCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC423230.140081309582673Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAA414080.13705282865579765No Hit
GGGAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC406520.1345506083490023TruSeq Adapter, Index 5 (97% over 35bp)
GGGGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAGATCG401490.13288577129302598No Hit
GCGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA399400.13219401991191457No Hit
CGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA397850.1316809985527171Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAGAT390000.1290827936045235No Hit
GGGGTCAGCGGAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA387380.12821562201671874Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA378630.12531953369866852No Hit
GGGAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA370160.12251612020679593Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAGA366070.12116240578155875No Hit
GGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC360230.11922947369271153TruSeq Adapter, Index 5 (97% over 35bp)
GGGCGCGGCGTGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTG355950.11781287277828238Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCCC347660.11506903596038111Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA347560.11503593780817484Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGTCAGCGGAGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCT345730.11443024162279976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA345440.11433425698140152No Hit
CGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT342320.11330159463256533Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC337430.11168309498967785Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT335680.11110387732606782Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA335100.11091190804327135No Hit
GGGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAGATCG333510.11038564742319136No Hit
GGGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTTGGTGGTAGTAGCAA332430.11002818737936346No Hit
GGGGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAGATCG316360.10470931431981294No Hit
TCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA316230.10466628672194478Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGCAGCGGAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC312610.10346813361207714Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC312500.10343172564465024No Hit
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA306070.1013035144577859Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGATATTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACA302800.10022120488064029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)