FastQCFastQC Report
Mon 25 Sept 2023
SRR24207638.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24207638.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32361765
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA528149516.320169805324277Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA24722207.63932375134669Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC17740025.48178382730361Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC14054874.343048038325475Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC9726533.005562273874741Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7041632.1759103683003693Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC6213651.9200590573474594Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4541861.4034648604611029Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4465231.37978568227042Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4167091.287658445081719Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA4106821.269034615386398Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA3699041.1430278910930847No Hit
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3324561.0273110876369074Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA3084910.9532576483390198No Hit
GGGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2554920.789487223580049Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2498040.7719109263663462Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT2352350.7268917501872967Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2348160.7255970124002816Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2132440.6589381018000718Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1942170.6001434099777933Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1624020.5018329500878582Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC1530270.472863578361687No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAA1520920.46997436635486356No Hit
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1462580.451946919458812Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1437200.44410433114510284Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAA1318430.407403613492651No Hit
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1291660.39913150596081515Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAG1243800.3843424485654599No Hit
GCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1195320.36936180705842214Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1193280.3687314335296607Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1053560.32555702694213373Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1046980.3235237633052462Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT1039840.32131745595458094Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC969310.2995232182175478Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAGA882710.27276324390835915No Hit
GGGGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTG839210.25932145542741564Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT819430.2532093042514832TruSeq Adapter, Index 1 (100% over 35bp)
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAA703370.2173459945710625No Hit
GGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA689180.2129611904665892Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA660270.2040278087428173Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA635270.19630264294917166Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA609350.18829319105431983Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCAAAAAAAAAAAAAAAGATCAGAAGAGCACACGTCTGAACTCCAGTCA598760.18502081082413152Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA575680.1778889377634378No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAG571870.1767116224964862No Hit
GGGAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT567810.17545705557159816TruSeq Adapter, Index 1 (100% over 35bp)
GGGGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAGATC557310.17221248593826696No Hit
CGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA546640.16891538517753898Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCT539620.16674615862268327Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT529760.16369935323366944TruSeq Adapter, Index 1 (100% over 35bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA524360.16203071742224195Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC472450.14599018316831608Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAA471140.14558538448072902No Hit
GGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC453940.1402704704147008Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAA426620.13182840923540481No Hit
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA416840.1288063243769306Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGTCGCGGCGTGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT410980.12699554551490005No Hit
GCGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA405260.12522802758131393Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGTAGTCAGCGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC395740.12228628444709366No Hit
CGTATGCCGTCTTCTGCTTGAAAGATCGGAAGAGCACACGTCTGAACTCC393750.12167136124991947Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAGA383750.11858129493246118No Hit
GAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA382210.11810542471957261Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC370090.1143602643428132Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA369390.11414395970059113Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAA339730.10497882300300988No Hit
CGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA336620.10401781237828035Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)