FastQCFastQC Report
Mon 25 Sept 2023
SRR24212099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24212099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40994652
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length84
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAACGTG3827260.9335998266310443No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAACGTG3274930.7988676181468743No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAACGTGAT2552720.6226958579865491No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAACGT2266600.5529013882103451No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAA2009600.49021028401460753No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAACGTGA2004890.4890613536614483No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAACGTGA1708580.41678119380059625No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAACGTGA1644500.4011498865754489No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAACGTG1634780.39877884559185917No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAAC1520770.37096790088619364No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAAAAAAAACGTG1193220.2910672348188246No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAAAAAAAAAAAAAAA1184450.28892793137992734No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAACGTG1166250.28448832789213574No Hit
GCTGAGAAGACGGTCGAACTTGACTATCAAAAAAAAAAAAAAACGTGATA1001450.24428796224444105No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAACGTG992260.2420462064173639No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAACGTGA976340.23816277303683417No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAACGTG865210.21105435899297303No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAACGTG847370.20670257183790702No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAAAAAAAACGT805590.19651099855659224No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAACGTGATA795100.1939521281946728No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAAA790860.1929178469425719No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAACGT768070.18735858521252965No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAACGT752260.18350198460033276No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAACGT741240.18081382908190072No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAAAAAAACGTG699290.17058078697679882No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAACGTGAT692510.1689269127104677No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAACGTGA671210.16373111302420618No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAACGTGA663220.1617820783062142No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAAAACGTGAT652550.15917929977793202No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAACGTGA604340.14741922922043588No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAA597490.14574827955607478No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAAA592060.14442371653746444No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCAAAAAAAAAAAAAAACGT550670.1343272776166023No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAACGT528960.12903146488473669No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCCAAAAAAAAAAAAAAACGTGA520910.12706779411129043No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGGAAAAAAAAAAAAAAACGTG518520.1264847912356958No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTTGAAAAAAAAAAAAAAACGTGA501660.12237205965304938No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAACGTG500620.12211836802517557No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAACGTGAT497780.12142559473367404No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAACGTGAT495590.12089137870959363No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAACGTGA494930.12073038209959679No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAAAAAAAAAAAAA469000.11440516680078171No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAACG449620.10967772088905645No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAAAAAAACGT437940.10682856876062762No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAACGT437440.10670660163184212No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTCAAAAAAAAAAAAAAACGTG428320.10448192120279494No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAACGTG423350.10326956794266726No Hit
GCTGAGAAGACGGTCGAACTTGACTATCCAAAAAAAAAAAAAAACGTGAT421840.1029012272137351No Hit
AAAAAAAAAAAAAAACGTGATAGATCGGAAGAGCGGTTCAGCAGGAATGC421530.1028256075938881Illumina Paired End PCR Primer 2 (100% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAACGTGA419880.10242311606889602No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAACG418780.10215478838556795No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAACGT413370.10083510405210903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)