FastQCFastQC Report
Sat 1 Jul 2023
SRR2442882.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2442882.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16759139
Total Bases854.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG167818510.013551412157868No Hit
ATGTATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAA8170624.8753220556258885No Hit
AGAAACGGATAGAGCGAATTCTGAGTGGTTGGAATTCTCGGGTGCCAAGG2228421.32967451370861Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGTGCTGAAGCTTATGTAGCCTTCATTCTGGAATTCTCGGGTGCCAAG1386430.8272680356669874No Hit
AAGCCTAGAATCAATTGTAAACGACTTTTGTTGGAATTCTCGGGTGCCAA1030780.6150554631714672No Hit
TGTATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAAG928880.5542528169257382No Hit
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATG901970.5381959061262037RNA PCR Primer, Index 16 (100% over 49bp)
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG891760.5321037077143402Illumina Small RNA Adapter 2 (100% over 21bp)
AGCCTAGAATCAATTGTAAACGACTTTTGTTGGAATTCTCGGGTGCCAAG734160.43806546386422357No Hit
TCGGGACGCAGGGCTTCGATGATCGCTCGGTTGGAATTCTCGGGTGCCAA617890.3686883914501813No Hit
ATGTTAGTTACTTGTTCCCCGGATAGTTTTGGAATTCTCGGGTGCCAAGG594020.35444541631882165Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGGAATTCTCGGGTGCCAA570950.34067979267908693No Hit
TCGGGACGCAGGGCTTCGATGATCGCTCGGTGGAATTCTCGGGTGCCAAG566760.3381796642417012No Hit
TTATAAGCATATGGTATATTATTGGATAAATGGAATTCTCGGGTGCCAAG551440.3290383831770833No Hit
CAGAGTTCTACAGTCCGACGATCATGGAATTCTCGGGTGCCAAGGAACTC550240.32832235593964587RNA PCR Primer, Index 1 (100% over 26bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA537210.3205474935198043No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTTGGAATTCTCGGGTGCCA481480.28729399523448074No Hit
ATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAAGGAA439570.26228674396697826RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA428900.25592006844743037RNA PCR Primer, Index 1 (100% over 22bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCTGGAATTCTCGGGTGCCAAG392550.23423041004672138No Hit
ATGGTGCTGAAGCTTATGTAGCCTTCATTCATGGAATTCTCGGGTGCCAA371570.22171186717885685No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCTGGAATTCTCGGGTGCCAAGG344630.20563705569838642Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGTTCTACAGTCCGACGATCATGGAATTCTCGGGTGCCAAGGAACTCC318870.19026633766806278RNA PCR Primer, Index 1 (100% over 27bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG312720.18659669807619592No Hit
ACGATTATCAATTAACAATCAATTCAGAACTTGGAATTCTCGGGTGCCAA296940.17718093990389364No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA294030.17544457385310783RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA283730.16929867339843652No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC274340.16369576026548857RNA PCR Primer, Index 16 (100% over 50bp)
CGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG256930.15330739842900043No Hit
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG253810.15144572761166308Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTATTTTTGATTTGAAAATTTGGTATTGGAATTCTCGGGTGCCAAGGA251410.15001367313678823RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG239780.1430741758272904No Hit
TTGTATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAA222240.13260824437341323No Hit
TTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAA219830.13117022300489303RNA PCR Primer, Index 1 (100% over 23bp)
GTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTA193560.11549519339865849RNA PCR Primer, Index 16 (100% over 47bp)
AGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG192470.11484480199131948No Hit
ATGTATGTAAGCGTATTACCGGTGGAGTTCTGGAATTCTCGGGTGCCAAG181450.1082692851941857No Hit
TTATAAGCATATGGTATATTATTGGATAAATTGGAATTCTCGGGTGCCAA179150.10689689965576393No Hit
AAACGGATAGAGCGAATTCTGAGTGGTTGGAATTCTCGGGTGCCAAGGAA176680.10542307692537188RNA PCR Primer, Index 1 (100% over 23bp)
GAAGCTTATGTAGCCTTCATTCATGTTGGCAGTGGAATTCTCGGGTGCCA175540.10474285104980631No Hit
CGGGACGCAGGGCTTCGATGATCGCTCGGTTGGAATTCTCGGGTGCCAAG172900.10316759112744395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)