FastQCFastQC Report
Sat 1 Jul 2023
SRR2442883.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2442883.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16612366
Total Bases847.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG167524210.084307075825322No Hit
ATGTATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAA8137184.898266748998909No Hit
AGAAACGGATAGAGCGAATTCTGAGTGGTTGGAATTCTCGGGTGCCAAGG2223791.3386353274422198Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGTGCTGAAGCTTATGTAGCCTTCATTCTGGAATTCTCGGGTGCCAAG1390040.8367501655092356No Hit
AAGCCTAGAATCAATTGTAAACGACTTTTGTTGGAATTCTCGGGTGCCAA1028200.6189365199394234No Hit
TGTATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAAG921930.5549661017581722No Hit
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATG899020.5411751703520137RNA PCR Primer, Index 16 (100% over 49bp)
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG886010.5333436549616111Illumina Small RNA Adapter 2 (100% over 21bp)
AGCCTAGAATCAATTGTAAACGACTTTTGTTGGAATTCTCGGGTGCCAAG733770.44170107978598594No Hit
TCGGGACGCAGGGCTTCGATGATCGCTCGGTTGGAATTCTCGGGTGCCAA618270.3721745596021662No Hit
ATGTTAGTTACTTGTTCCCCGGATAGTTTTGGAATTCTCGGGTGCCAAGG594580.35791409844931177Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGGACGCAGGGCTTCGATGATCGCTCGGTGGAATTCTCGGGTGCCAAG566060.3407461646342249No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGGAATTCTCGGGTGCCAA563640.3392894184970401No Hit
CAGAGTTCTACAGTCCGACGATCATGGAATTCTCGGGTGCCAAGGAACTC549090.33053088283751997RNA PCR Primer, Index 1 (100% over 26bp)
TTATAAGCATATGGTATATTATTGGATAAATGGAATTCTCGGGTGCCAAG544040.3274909787082707No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA532130.32032162065295217No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTTGGAATTCTCGGGTGCCA480560.289278480861787No Hit
ATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAAGGAA436440.26271995211278154RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA431090.25949945961941845RNA PCR Primer, Index 1 (100% over 22bp)
ATAATTTTGAACGAAAGGGAATACGGTTCCTGGAATTCTCGGGTGCCAAG389570.23450603002606613No Hit
ATGGTGCTGAAGCTTATGTAGCCTTCATTCATGGAATTCTCGGGTGCCAA372670.22433288551432107No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCTGGAATTCTCGGGTGCCAAGG340300.2048474010264402Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGTTCTACAGTCCGACGATCATGGAATTCTCGGGTGCCAAGGAACTCC320280.19279613752791144RNA PCR Primer, Index 1 (100% over 27bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG311210.18733634931953702No Hit
ACGATTATCAATTAACAATCAATTCAGAACTTGGAATTCTCGGGTGCCAA295780.17804808779194967No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA293820.17686824381307273RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA284190.1710713573250192No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC275250.16568982407442745RNA PCR Primer, Index 16 (100% over 50bp)
ATGTATTTTTGATTTGAAAATTTGGTATTGGAATTCTCGGGTGCCAAGGA250510.15079730364717464RNA PCR Primer, Index 1 (100% over 22bp)
CGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG249820.15038195040971286No Hit
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG244990.1474744777474804Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG239600.14422990680556882No Hit
TTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAA222260.13379189935979016RNA PCR Primer, Index 1 (100% over 23bp)
TTGTATTTTTGATTTGAAAATTTGGTATAACTGGAATTCTCGGGTGCCAA219930.13238932973183953No Hit
AGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG195190.11749680930458672No Hit
GTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTA194370.11700320110934227RNA PCR Primer, Index 16 (100% over 47bp)
ATGTATGTAAGCGTATTACCGGTGGAGTTCTGGAATTCTCGGGTGCCAAG184440.11102572625717493No Hit
GAAGCTTATGTAGCCTTCATTCATGTTGGCAGTGGAATTCTCGGGTGCCA176430.10620401693533602No Hit
TTATAAGCATATGGTATATTATTGGATAAATTGGAATTCTCGGGTGCCAA175760.10580070292214848No Hit
CGGGACGCAGGGCTTCGATGATCGCTCGGTTGGAATTCTCGGGTGCCAAG172830.1040369565659702No Hit
AAACGGATAGAGCGAATTCTGAGTGGTTGGAATTCTCGGGTGCCAAGGAA172560.10387442703826774RNA PCR Primer, Index 1 (100% over 23bp)
ATGGTGCTGAAGCTTATGTAGCCTTCATTCATTGGAATTCTCGGGTGCCA167590.10088267980611551No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)