FastQCFastQC Report
Sat 1 Jul 2023
SRR2442893.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2442893.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18481521
Total Bases942.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG826105944.69902125479824No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT17135419.271644904118011RNA PCR Primer, Index 1 (100% over 25bp)
ATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAA6443563.486487935706158RNA PCR Primer, Index 1 (100% over 23bp)
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG4778352.5854744314604843No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG2759141.4929182506136804No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG2470391.336681109741996Illumina Small RNA Adapter 2 (100% over 21bp)
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC2373721.2843748087616815RNA PCR Primer, Index 1 (100% over 24bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA2001411.0829249389160123RNA PCR Primer, Index 1 (100% over 22bp)
TGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAAC1362150.7370334941588411RNA PCR Primer, Index 1 (100% over 24bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA1352530.731828294868155No Hit
ATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1271550.6880115548931282RNA PCR Primer, Index 6 (100% over 34bp)
TGCTTGGACTACATATTGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1251920.6773901347188903No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG1139130.6163616079001291Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTTGGACTACATATGGTTGAGGGTTGTCTGGAATTCTCGGGTGCCAAG917720.4965608620632469No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA818470.4428585720839751No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA803480.43474776778383123RNA PCR Primer, Index 1 (100% over 22bp)
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA774190.4188995050786134RNA PCR Primer, Index 1 (100% over 32bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA751180.4064492310995399RNA PCR Primer, Index 1 (100% over 23bp)
AGGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT685220.3707595278548773RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA664960.3597972266460104No Hit
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG524700.2839052045554043Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAAC522630.2827851668701943RNA PCR Primer, Index 1 (100% over 24bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTTCCAAG508360.2750639408953408No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA420160.22734059604726256RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCTGGTGCCAAG416600.2254143476611043No Hit
TGCTTGGACTACATATGGTTGATGGTTGTATGGAATTCTCGGGTGCCAAG407750.22062578074607603No Hit
TCCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG406640.22002518082791994No Hit
TATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGA347110.18781462846050387RNA PCR Primer, Index 1 (100% over 22bp)
ACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCAAGGA332550.17993648899351952RNA PCR Primer, Index 1 (100% over 22bp)
ATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG331740.1794982133775678RNA PCR Primer, Index 1 (100% over 29bp)
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT326910.17688479211207778RNA PCR Primer, Index 1 (100% over 30bp)
TTCTTGGACTACATATTGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG324270.1754563382526795No Hit
ACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCC317910.17201506304594735RNA PCR Primer, Index 1 (100% over 27bp)
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC316180.1710789929032356RNA PCR Primer, Index 1 (100% over 26bp)
NGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG313770.16977498767552734No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG286140.15482491944250693Illumina Small RNA Adapter 2 (100% over 21bp)
TACTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG245890.13304640889675695No Hit
AAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC239740.1297187607015678RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGA227780.12324743185368779RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTTGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG224970.12172699422304041No Hit
TGCTTGGACTACATATGGTTGAGTGTTGTATGGAATTCTCGGGTGCCAAG214470.11604564364588824No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGACTTCTCGGGTGCCAAGGAACT206550.11176028206769345RNA PCR Primer, Index 1 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)