FastQCFastQC Report
Sat 1 Jul 2023
SRR2442894.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2442894.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18376555
Total Bases937.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG843023145.87492595864677No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT16974019.236774792663805RNA PCR Primer, Index 1 (100% over 25bp)
ATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAA6369003.4658291502406193RNA PCR Primer, Index 1 (100% over 23bp)
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG4590012.497753251357504No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG2772811.50888455425949No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG2488121.3539643311817693Illumina Small RNA Adapter 2 (100% over 21bp)
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC2374521.2921464333222414RNA PCR Primer, Index 1 (100% over 24bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA2007931.0926585532489632RNA PCR Primer, Index 1 (100% over 22bp)
TGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAAC1361340.7408026150712144RNA PCR Primer, Index 1 (100% over 24bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA1335110.726528993056642No Hit
ATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1244030.6769658404418021RNA PCR Primer, Index 6 (100% over 34bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG1133620.6168838501013928Illumina Small RNA Adapter 2 (100% over 21bp)
AGGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT848240.461588148594772RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA821500.447036999045795No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA808840.44014778613292865RNA PCR Primer, Index 1 (100% over 22bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA759460.41327659074293305RNA PCR Primer, Index 1 (100% over 23bp)
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA757880.41241679955791494RNA PCR Primer, Index 1 (100% over 32bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA669170.36414333372060215No Hit
TCCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG562460.3060747784337162No Hit
TGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAAC530900.28890072159879804RNA PCR Primer, Index 1 (100% over 24bp)
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG523220.28472148343364684Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTTCCAAG471430.25653883440068065No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTCTGGAATTCTCGGGTGCCAAG432540.23537599947324184No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA422380.2298472156505939RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCTGGTGCCAAG421940.22960778013071548No Hit
TGCTTGGACTACATATGGTTGATGGTTGTATGGAATTCTCGGGTGCCAAG419400.22822558417505348No Hit
TACTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG353410.19231569790964628No Hit
TATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGA346690.188658864515139RNA PCR Primer, Index 1 (100% over 22bp)
ACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCAAGGA334820.18219954719478162RNA PCR Primer, Index 1 (100% over 22bp)
ATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG331340.18030582990119748RNA PCR Primer, Index 1 (100% over 29bp)
AGGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAA324690.17668708852121628RNA PCR Primer, Index 1 (100% over 23bp)
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT324100.17636602725592473RNA PCR Primer, Index 1 (100% over 30bp)
ACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCC320050.1741621321297708RNA PCR Primer, Index 1 (100% over 27bp)
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC314030.17088621888052466RNA PCR Primer, Index 1 (100% over 26bp)
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG289510.15754313036366174Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTTGGACTACATATTGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG256840.13976504301268655No Hit
AAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC238490.12977949349048284RNA PCR Primer, Index 1 (100% over 33bp)
TGCTTTGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG230450.1254043535363402No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGA226970.12351063624275606RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTGGACTACATATGGTTGAGTGTTGTATGGAATTCTCGGGTGCCAAG219340.11935860665940924No Hit
NGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG198010.1077514256616651No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAT190160.10347967831837904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)