FastQCFastQC Report
Sat 1 Jul 2023
SRR2442916.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2442916.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15236919
Total Bases777 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG669355943.92987191176904No Hit
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG3376032.215690718051333No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA2764521.8143563012968698No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG2213451.4526886964484094No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG2072791.3603734455764975Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG2020841.3262786262760864Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA1271990.8348078768417684RNA PCR Primer, Index 1 (100% over 22bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA1264010.8295705975729083RNA PCR Primer, Index 1 (100% over 22bp)
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG1150490.7550673466204028Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTTGGACTACATATTGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG968280.6354828033147646No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA749520.49191047087669104RNA PCR Primer, Index 1 (100% over 23bp)
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT711230.4667807185954063RNA PCR Primer, Index 1 (100% over 25bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG671970.4410143546736712No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA635110.4168231123365557No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG564660.3705867308213688No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTCTGGAATTCTCGGGTGCCAAG562430.36912318034899316No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG555580.3646275208262248Illumina Small RNA Adapter 2 (100% over 21bp)
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA540380.35465175079030087RNA PCR Primer, Index 1 (100% over 32bp)
ATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAA533640.3502282843401609RNA PCR Primer, Index 1 (100% over 23bp)
ATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG527210.346008271094701RNA PCR Primer, Index 6 (100% over 34bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA516390.3389070979507078RNA PCR Primer, Index 1 (100% over 22bp)
TAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA423790.27813365681080277RNA PCR Primer, Index 1 (100% over 22bp)
ACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCC390510.2562919708374114RNA PCR Primer, Index 1 (100% over 27bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTTCCAAG343910.2257083600693815No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCTGGTGCCAAG316500.2077191589717055No Hit
TGCTTGGACTACATATGGTTGATGGTTGTATGGAATTCTCGGGTGCCAAG307570.2018583940756002No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA291210.19112131527377682No Hit
TCCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG286740.18818765132242285No Hit
TGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGG283770.18623843836145615Illumina Small RNA Adapter 2 (100% over 21bp)
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC281700.18487989599472177RNA PCR Primer, Index 1 (100% over 24bp)
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT279650.18353447964119257RNA PCR Primer, Index 1 (100% over 30bp)
NGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG259760.1704806595086579No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAA242640.1592447922050383No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA240700.1579715689241375No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGA224070.14705728894404438RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTTGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG220670.14482586669916667No Hit
ATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG214970.1410849529356952Illumina Small RNA Adapter 2 (100% over 21bp)
ATGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA211950.13910292494171558No Hit
TGCTTGGACTACATATGGTTGAGTGTTGTATGGAATTCTCGGGTGCCAAG205520.13488291169625566No Hit
GACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTC196130.1287202484964316RNA PCR Primer, Index 1 (100% over 26bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTAGGGTGCCAAG188000.1233845241285328No Hit
TGCTTGTACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG184910.12135655508833511No Hit
TTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAA182080.11949922421980455RNA PCR Primer, Index 1 (100% over 23bp)
TACTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG179450.11777315348332561No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGTAATTCTCGGGTGCCAAG175630.11526608496113945No Hit
TATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAG172840.11343500611901922No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATTGAATTCTCGGGTGCCAAG167510.10993692359984326No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAT162060.10636008500143632No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGTGTGCCAAG161260.10583504447322979No Hit
CTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGA157550.10340016902367205RNA PCR Primer, Index 1 (100% over 22bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGG156590.10277012038982422Illumina Small RNA Adapter 2 (100% over 21bp)
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA156490.10270449032379839RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCAAAG155830.10227133188802802No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG153430.10069621030340845Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)