FastQCFastQC Report
Sat 1 Jul 2023
SRR2442917.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2442917.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15161915
Total Bases773.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG681048444.91836288489943No Hit
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG3216492.1214272735337194No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA2742611.8088810021689212No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG2194401.447310580490657No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG2062601.3603822472293243Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG2024941.335543696162391Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA1273040.8396300863050611RNA PCR Primer, Index 1 (100% over 22bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA1265780.8348417729554611RNA PCR Primer, Index 1 (100% over 22bp)
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG1141260.752714943989595Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA750120.4947396156751967RNA PCR Primer, Index 1 (100% over 23bp)
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT705320.4651918969338636RNA PCR Primer, Index 1 (100% over 25bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG672740.4437038461170637No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA639740.4219387854370638No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG562240.37082387020373087Illumina Small RNA Adapter 2 (100% over 21bp)
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG555180.36616746631279756No Hit
ATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAA528940.34886094533573103RNA PCR Primer, Index 1 (100% over 23bp)
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA527600.3479771519626643RNA PCR Primer, Index 1 (100% over 32bp)
ATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG520950.3435911624619977RNA PCR Primer, Index 6 (100% over 34bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA514030.3390270951921311RNA PCR Primer, Index 1 (100% over 22bp)
TAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA423790.27950954744173145RNA PCR Primer, Index 1 (100% over 22bp)
TCCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG395960.2611543462682649No Hit
ACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCC393880.2597824878981316RNA PCR Primer, Index 1 (100% over 27bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCTGGTGCCAAG323740.2135218407437319No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTTCCAAG323020.2130469666925319No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTCTGGAATTCTCGGGTGCCAAG315820.20829822618053193No Hit
TGCTTGGACTACATATGGTTGATGGTTGTATGGAATTCTCGGGTGCCAAG311650.20554791396733196No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA291630.19234377715479872No Hit
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT283440.18694208482239874RNA PCR Primer, Index 1 (100% over 30bp)
TGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGG279170.18412581787986543Illumina Small RNA Adapter 2 (100% over 21bp)
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC277790.18321564261506545RNA PCR Primer, Index 1 (100% over 24bp)
TGCTTGGACTACATATTGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG268370.17700270711186547No Hit
TACTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG253730.16734693473746554No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA243520.16061295687253227No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAA241650.15937960343399893No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGA227680.15016572774613232RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTTGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG226470.149367675521199No Hit
ATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG214140.14123545739439908Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTTGGACTACATATGGTTGAGTGTTGTATGGAATTCTCGGGTGCCAAG210270.13868300936919908No Hit
ATGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA206920.13647352593653242No Hit
GACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTC195530.1289612822654658RNA PCR Primer, Index 1 (100% over 26bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTAGGGTGCCAAG195110.12868427240226582No Hit
TGCTTGTACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG187070.12338151216386584No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGTAATTCTCGGGTGCCAAG179450.11835576178866587No Hit
TTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAA178050.11743239557799921RNA PCR Primer, Index 1 (100% over 23bp)
TATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAG172380.11369276242479925No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATTGAATTCTCGGGTGCCAAG169800.11199113040799924No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAT165550.10918805441133261No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGTGTGCCAAG164670.10860765279319927No Hit
NGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG162230.10699835739746595No Hit
CTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGA160590.10591669983639929RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCAAAG156210.1030278826915993No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA154760.102071539116266RNA PCR Primer, Index 1 (100% over 22bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGG153080.10096349966346599Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG152920.10085797209653266Illumina Small RNA Adapter 2 (100% over 21bp)
CATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC151880.100172042911466RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)