FastQCFastQC Report
Sat 1 Jul 2023
SRR2442924.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2442924.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13029212
Total Bases664.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG747245357.351534382892844No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA2617822.00919288135No Hit
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1955041.5005051725307716No Hit
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG1159970.8902840785766629Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG1092890.8387997677833471No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGTGCCAAGG1026530.7878680613992619No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG927470.7118389047626211Illumina Small RNA Adapter 2 (100% over 21bp)
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA727790.5585832819360066RNA PCR Primer, Index 1 (100% over 32bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAGG573360.4400573112172862No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCAAGG561730.43113121499596446No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA459590.352738139497615No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA422390.32418691168736835No Hit
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCA384940.29544380734613884No Hit
ATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA383930.2946686261609681RNA PCR Primer, Index 1 (100% over 34bp)
CATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC348700.2676293854148662RNA PCR Primer, Index 1 (100% over 31bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG346400.26586412132982407Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTGGGTGCCAAGG332040.2548427333901697No Hit
TGCTTGGACTACATATGGTTGAGGTTTGTATGGAATTCTCGGGTGCCAAG319780.24543310831077123No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCCGGGTGCCAAGG307600.2360848837212872No Hit
TTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAA268660.20619819525539992RNA PCR Primer, Index 1 (100% over 23bp)
TATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGA256930.19719534842168504RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGGCCAAGG239420.1837563161916469No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTTCGGGTGCCAAGG227370.17450786739827398No Hit
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT218560.16774613844643865RNA PCR Primer, Index 1 (100% over 30bp)
CTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGA212690.16324087749896155RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGACTTCTCGGGTGCCAAG199470.15309444654058893No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTCCAAGG198220.15213506388567474No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG195820.1502930491882395Illumina Small RNA Adapter 2 (100% over 21bp)
ATGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA167420.12849587526858877No Hit
TATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC151970.11663790565384921RNA PCR Primer, Index 1 (100% over 33bp)
NGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG147560.11325320364731191No Hit
ATGTATGTAAGCGTATTACCGGTGGAGTTCTGGAATTCTCGGGTGCCAAG140390.10775018473872403No Hit
TCTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCA130650.10027467509163256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)