FastQCFastQC Report
Sat 1 Jul 2023
SRR2442925.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2442925.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13366693
Total Bases681.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG768579257.49957749459795No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA2711242.0283551062330827No Hit
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1680691.2573715877218097No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG1159370.8673573934854344No Hit
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG1121780.8392352543744366Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGTGCCAAGG1054550.7889385953578795No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG957940.716661929768268Illumina Small RNA Adapter 2 (100% over 21bp)
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA758430.5674028721988303RNA PCR Primer, Index 1 (100% over 32bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAGG584820.437520335059689No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCAAGG574900.4300989032964249No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA481940.3605529056439016No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA442530.3310691732053695No Hit
ATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA402630.30121885794788583RNA PCR Primer, Index 1 (100% over 34bp)
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCA397430.2973285912977877No Hit
CATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC362790.2714134303825187RNA PCR Primer, Index 1 (100% over 31bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG352900.2640144424653128Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTGGGTGCCAAGG340320.25460298968488315No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCCGGGTGCCAAGG312950.23412672079773209No Hit
TTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAA279850.20936367731345368RNA PCR Primer, Index 1 (100% over 23bp)
TATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGA269300.20147092478296613RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGGCCAAGG248690.18605200254094265No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTTCGGGTGCCAAGG237650.17779266719150352No Hit
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT225250.1685158774874234RNA PCR Primer, Index 1 (100% over 30bp)
CTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGA221010.16534381391118955RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTGGACTAAATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG206480.15447351113697308No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG206170.15424159139437107Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTCCAAGG204560.15303710498924455No Hit
ATGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA175540.13132642456888927No Hit
TATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC159760.11952096154224534RNA PCR Primer, Index 1 (100% over 33bp)
ATGTATGTAAGCGTATTACCGGTGGAGTTCTGGAATTCTCGGGTGCCAAG143470.10733395313261103No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATGCTCGGGTGCCAAG137930.10318932289385264No Hit
TCTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCA137340.10274792725470691No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)