FastQCFastQC Report
Sat 1 Jul 2023
SRR2442929.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2442929.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11844174
Total Bases604 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG391294933.03690911666782No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT8102316.840755632262748RNA PCR Primer, Index 1 (100% over 25bp)
ATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC5923905.00153071037288RNA PCR Primer, Index 2 (100% over 34bp)
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG5530234.669156329516942No Hit
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2690382.2714796320959145RNA PCR Primer, Index 1 (100% over 32bp)
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT1312691.1083001651275979RNA PCR Primer, Index 1 (100% over 30bp)
AAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1270691.072839693168979RNA PCR Primer, Index 1 (100% over 33bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA1035830.8745481111641893No Hit
ATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC1013380.8555936446053563RNA PCR Primer, Index 16 (100% over 39bp)
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC1006480.849767995640726RNA PCR Primer, Index 1 (100% over 26bp)
ATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCC889680.7511541117177103No Hit
TGCTGGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG702060.5927471176968525No Hit
ATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG664280.5608495788731236RNA PCR Primer, Index 1 (100% over 29bp)
TGCTCGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG626070.5285889923602947No Hit
AGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC618710.5223749667980224RNA PCR Primer, Index 1 (100% over 27bp)
ATGTATGTAAGCGTATTACCGGTGTGGAATTCTCGGGTGCCAAGGAACTC470970.3976385351988243RNA PCR Primer, Index 1 (100% over 26bp)
TTCTCGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG435230.36746336215594266No Hit
TGCTAGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG418490.35332983118957895No Hit
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATG366540.30946860456457326RNA PCR Primer, Index 16 (100% over 49bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA351160.2964833174521077No Hit
ACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC316510.2672284280862473RNA PCR Primer, Index 2 (100% over 34bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG314050.26515145758581393Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA310600.2622386331034988RNA PCR Primer, Index 1 (100% over 22bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG297280.2509925977109083Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTTTGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT272350.22994427471261397RNA PCR Primer, Index 1 (100% over 25bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG271390.22913374963927413No Hit
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG260490.2199309128690612Illumina Small RNA Adapter 2 (100% over 21bp)
ACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCC254300.21470471473992192RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA228540.19295562527196916No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA201380.17002452007206242RNA PCR Primer, Index 1 (100% over 22bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG201330.16998230522449265No Hit
GTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT192100.1621894443631105RNA PCR Primer, Index 16 (100% over 37bp)
GGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG183020.15452322804443772RNA PCR Primer, Index 16 (100% over 36bp)
ATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAA181080.15288529195873007RNA PCR Primer, Index 1 (100% over 23bp)
TGCTTGGACTACATATTGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG179390.1514584301108714No Hit
AGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA166620.14067675804154853RNA PCR Primer, Index 1 (100% over 28bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA166520.14059232834640895RNA PCR Primer, Index 1 (100% over 23bp)
GGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACT165660.13986623296820866RNA PCR Primer, Index 1 (100% over 25bp)
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG160360.13539145912581155Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGTAATTCTCGGGTGCCAAG158680.1339730402474668No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA153770.12982754221611403No Hit
ATGGGTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC153350.12947293749652783RNA PCR Primer, Index 2 (100% over 34bp)
GACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTC149760.12644191144101732RNA PCR Primer, Index 1 (100% over 26bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCTGGTGCCAAG148780.12561450042864955No Hit
ATGGCTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC147290.12435649797106998RNA PCR Primer, Index 2 (100% over 34bp)
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG141340.11933293111026567No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA140850.1189192256040818RNA PCR Primer, Index 1 (100% over 22bp)
AGGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC139360.11766122314650224RNA PCR Primer, Index 2 (100% over 34bp)
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC138580.11700267152441361RNA PCR Primer, Index 1 (100% over 24bp)
ATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCA136230.11501857368863375No Hit
TTTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC135130.1140898470420985RNA PCR Primer, Index 1 (100% over 26bp)
CGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC134580.11362548371883088RNA PCR Primer, Index 1 (100% over 31bp)
AACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCCAAGGAACTCC129190.10907472315080816RNA PCR Primer, Index 1 (100% over 27bp)
TGCTTGGACTACATATGGTTGATGGTTGTATGGAATTCTCGGGTGCCAAG125570.10601836818675578No Hit
GAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCA123840.10455773446084125RNA PCR Primer, Index 16 (100% over 40bp)
TGCTTGTACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG123620.10437198913153421No Hit
TGCTTTGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG123560.10432133131445046No Hit
ACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC122010.10301267103978715RNA PCR Primer, Index 1 (100% over 33bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA119450.1008512708442142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)