FastQCFastQC Report
Sat 1 Jul 2023
SRR2442930.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2442930.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12175465
Total Bases620.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG401517032.977549522749236No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT8447726.938314060284351RNA PCR Primer, Index 1 (100% over 25bp)
ATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC6179985.07576507344894RNA PCR Primer, Index 2 (100% over 34bp)
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG6088425.00056466015877No Hit
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2800612.3002078360046205RNA PCR Primer, Index 1 (100% over 32bp)
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT1380251.133632267843569RNA PCR Primer, Index 1 (100% over 30bp)
AAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1325431.088607293438074RNA PCR Primer, Index 1 (100% over 33bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA1093990.898520097589702No Hit
ATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC1050260.862603604872586RNA PCR Primer, Index 16 (100% over 39bp)
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC1039170.8534951231842068RNA PCR Primer, Index 1 (100% over 26bp)
ATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCC923690.7586486429881734No Hit
ATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG694700.5705736906146911RNA PCR Primer, Index 1 (100% over 29bp)
AGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC642700.5278648495149877RNA PCR Primer, Index 1 (100% over 27bp)
TGCTGGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG627700.515544991505458No Hit
TGCTCGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG588030.482963073689588No Hit
ATGTATGTAAGCGTATTACCGGTGTGGAATTCTCGGGTGCCAAGGAACTC488770.4014384666211927RNA PCR Primer, Index 1 (100% over 26bp)
TTCTCGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG399550.32815995118050933No Hit
TGCTAGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG399500.32811888498714425No Hit
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATG384380.3157004681135382RNA PCR Primer, Index 16 (100% over 49bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA362570.2977873945676818No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG327740.26918068426955355Illumina Small RNA Adapter 2 (100% over 21bp)
ACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC326670.26830186773154047RNA PCR Primer, Index 2 (100% over 34bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA320240.26302075526478863RNA PCR Primer, Index 1 (100% over 22bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG315480.25911125365643123Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG280520.23039777125555366No Hit
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG267650.21982733308337712Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTTTGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT264360.21712517755995356RNA PCR Primer, Index 1 (100% over 25bp)
ACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCC262810.21585212556563546RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA234900.19292897642923698No Hit
AGGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT218650.1795824635855797RNA PCR Primer, Index 1 (100% over 25bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG211200.17346360077417988No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA207380.17032614360108628RNA PCR Primer, Index 1 (100% over 22bp)
GTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT199030.16346808930911466RNA PCR Primer, Index 16 (100% over 37bp)
GGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG190910.15679893950662255RNA PCR Primer, Index 16 (100% over 36bp)
ATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAA187670.1541378501765641RNA PCR Primer, Index 1 (100% over 23bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA174880.14363311791377167RNA PCR Primer, Index 1 (100% over 23bp)
AGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA170620.14013427823906519RNA PCR Primer, Index 1 (100% over 28bp)
GGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACT169750.13941972647451248RNA PCR Primer, Index 1 (100% over 25bp)
TGCTTGGACTACATATTGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG168020.13799883618408004No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG163890.1346067686121228Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA159390.13091081120926387No Hit
GACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTC158370.13007306086461587RNA PCR Primer, Index 1 (100% over 26bp)
TTTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC154830.1271655743743668RNA PCR Primer, Index 1 (100% over 26bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCTGGTGCCAAG154280.12671384624735071No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGTAATTCTCGGGTGCCAAG152410.125177970615496No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG148230.12174483685017368No Hit
TATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAAC145530.11952726240845833RNA PCR Primer, Index 1 (100% over 24bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA145400.11942049030570906RNA PCR Primer, Index 1 (100% over 22bp)
ATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCA142090.11670190830493948No Hit
CGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC140160.11511675324104664RNA PCR Primer, Index 1 (100% over 31bp)
ATGGCTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC135720.11147007527022582RNA PCR Primer, Index 2 (100% over 34bp)
AACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCCAAGGAACTCC133950.11001633202510129RNA PCR Primer, Index 1 (100% over 27bp)
ATGGGTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC133420.10958103037543125RNA PCR Primer, Index 2 (100% over 34bp)
TGCTTGGACTACATATGGTTGATGGTTGTATGGAATTCTCGGGTGCCAAG128950.10590971268859137No Hit
TGCCTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG128940.10590149944991833No Hit
GAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCA127430.10466130041029234RNA PCR Primer, Index 16 (100% over 40bp)
TGCTTGTACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG126650.10402066779379678No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA125670.10321577040384085No Hit
ACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC125620.10317470421047574RNA PCR Primer, Index 1 (100% over 33bp)
TGCTTTGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG123280.1012528063609891No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)