FastQCFastQC Report
Tue 26 Sept 2023
SRR24451158.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24451158.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119883640
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length1-132
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
A46175613.8517023673955846No Hit
ATAGATGGATACGAATAAGGCGTC33945182.8315106214659482No Hit
ATATAGATGGATACGAATAAGGCGTC24224142.020637678335426No Hit
ATTGTGGCGTCGCTGAACCATAG17613341.4692029704803757No Hit
AATAAATCATTTGTATACGACTTAGAT17315661.4443722262687386No Hit
ATTATAGATGGATACGAATAAGGCGTC15782281.3164665337155261No Hit
ATTGTGGCGTCGCTGAACCATA15195381.2675107295707737No Hit
AAGATGGATACGAATAAGGCGTC14438651.2043886888986688No Hit
ATCTATGCCGACTAGGGATCGGGTGGTG14076381.1741702203903719No Hit
AATAGATGGATACGAATAAGGCGTC13983441.1664177030327074No Hit
ATGTGGCGTCGCTGAACCATAG13749731.1469229663029918No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT12961821.081200070334868No Hit
ATTAGATGGATACGAATAAGGCGTC11346330.9464452363975602No Hit
AATATAGATGGATACGAATAAGGCGTC10411570.8684729626160834No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATT10307630.8598028888679057No Hit
ATTTTGATGATTCATAATAACTTTTCGAATCGCA10260310.8558557281043518No Hit
ATGTGGCGTCGCTGAACCATA9494050.7919387499411931No Hit
ATCCAGGGGGCATGCCTGTTTGAGCGTCATT8544760.7127544675820654No Hit
AATGATTAATAGGGACGGTCGGGGGC8277060.6904244816056637No Hit
ACTATGCCGACTAGGGATCGGGTGGTG8023890.6693065042069126No Hit
ATTTTGATGATTCATAATAACTTTTCGAATCGCAT7387050.6161849940492298No Hit
AAAATATAGATGGATACGAATAAGGCGTC6918650.5771137746568256No Hit
AACTATGCCGACTAGGGATCGGGTGGTG6809260.5679890934242571No Hit
ATATATAGATGGATACGAATAAGGCGTC6459490.5388133026324525No Hit
ATTGATGATTCATAATAACTTTTCGAATCGCA5996490.5001925200135732No Hit
ATTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCA5068940.4228216627389692No Hit
ATTGATGATTCATAATAACTTTTCGAATCGCAT5051950.42140445518671266No Hit
ATTTGATGATTCATAATAACTTTTCGAATCGCA4828750.402786401881024No Hit
AGCCAAGGACGTTTTCATTAATCAAGAACG4484170.3740435308771072No Hit
ATGCCAAGGACGTTTTCATTAATCAAGAACGAA4327580.36098169858706325No Hit
ATTGCCAAGGACGTTTTCATTAATCAAGAACGAA3930400.3278512397521463No Hit
ATTTGATGATTCATAATAACTTTTCGAATCGCAT3896640.3250351757754436No Hit
ATTGACCTCAAATCAGGTAGGAGTACCCGCTGAA3767370.3142522199025655No Hit
AATGATTAATAGGGACGGTCGGGGGCA3609660.3010969636891239No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT3579500.298581190894771No Hit
AGTGGCGTCGCTGAACCATAG3408670.28433154015009887No Hit
AACCATACGCGAAACTCAGGTGCTGCAATCT3258110.27177269559049094No Hit
AGTGGCGTCGCTGAACCATA3056250.254934701682398No Hit
ATTTCCAGGGGGCATGCCTGTTTGAGCGTCATT3045730.254057184116198No Hit
ATCCATCTAAAGCTAAATA3013690.2513845925932846No Hit
AGCCAAGGACGTTTTCATTAATCAAGAAC2783280.23216512278072302No Hit
AAATAGATGGATACGAATAAGGCGTC2747800.22920558635023094No Hit
ATCAATATAGATGGATACGAATAAGGCGTC2693040.22463782380982095No Hit
ATTGACCTCAAATCAGGTAGGAGTACCCGCTGAACT2656400.22158152688723834No Hit
ACCTATGCCGACTAGGGATCGGGTGGTG2640900.2202886065187877No Hit
ATTTCCATCTAAAGCTAAATA2377800.19834232594205517No Hit
ATTTCCAGGGGGCATGCCTGTTTGAGCGTCATTT2360800.19692428424762545No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT2267740.18916175718388265No Hit
ATTGACCTCAAATCAGGTAGGAGTACCCGCTGA2196800.1832443526072448No Hit
ATTGGGCCAGCATCAGTTTTGGTGGCAGGATA2114840.17640772335574728No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGG2057500.17162475213465322No Hit
AATGTTAGGGATAACAGGGTAATGCGA2036770.1698955754096222No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAAT2027690.1691381743163621No Hit
ACCAGGGGGCATGCCTGTTTGAGCGTCATT2017480.1682865151575311No Hit
ATCCAGGGGGCATGCCTGTTTGAGCGTCATTT1982990.1654095588021852No Hit
ACCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCA1956770.1632224380240707No Hit
ATTTGCTCGAATATATTAGCATGGAATAATAGAAT1945130.16225149653447293No Hit
AT1940590.16187279598784288No Hit
ATACTTATTCAATGAAGCGGAG1782300.148669159528356No Hit
ATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCA1707890.14246230761761988No Hit
AACTTTCAACAACGGATCTCTTGGTTCTCGCATCG1674480.13967543861697893No Hit
ATCTAAGTCAGAATCCATGCTAGAACGCGGTGATT1667070.1390573392666422No Hit
ATTGACCTCAAATCAGGTAGGAGTACCCGCTGAAC1657670.1382732456238399No Hit
ATCTAAGTCAGAATCCATGCTAGAACGCGGTGATTT1648790.1375325273740437No Hit
AATGATTAATAGGGACGGTCGGGGG1630530.13600938376579155No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG1622820.13536626015025904No Hit
AGCCAAGGACGTTTTCATTAATCAAGAACGA1608900.13420513424517308No Hit
ATTGGCGTCGCTGAACCATAG1578060.13163263978304296No Hit
ATTTCCATCTAAAGCTAAATATT1563550.13042229948973855No Hit
ATTACTTATTCAATGAAGCGGAG1539650.12842869969580503No Hit
AATGGATACGAATAAGGCGTC1516890.12653019210961564No Hit
ATAGATGGATACGAATAAGGCGT1411540.11774250431501745No Hit
AATTCCAGGGGGCATGCCTGTTTGAGC1390570.11599330817782975No Hit
ATGGCGTCGCTGAACCATAG1384440.11548197902566189No Hit
AGATGGATACGAATAAGGCGTC1376330.1148054897232016No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAA1361710.11358597386599205No Hit
ATTTGCTCGAATATATTAGCATGGAATAATAGAA1337980.11160655448900283No Hit
AAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTG1323930.11043458473566535No Hit
AAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGA1301910.10859780367029229No Hit
AATGTACACTAGGGATAACAGGGTAATCAACGCGA1284640.10715724013718635No Hit
AAAGTCAGAATCCATGCTAGAACGCGGTGATTT1243050.10368804283887277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)