FastQCFastQC Report
Mon 25 Sept 2023
SRR24451159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24451159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88447309
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length1-133
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGATGGATACGAATAAGGCGTC32941643.724436658666461No Hit
ATATAGATGGATACGAATAAGGCGTC21677262.450867103260315No Hit
AATAAATCATTTGTATACGACTTAGAT20315702.29692686297556No Hit
AAGATGGATACGAATAAGGCGTC14502361.639660964699333No Hit
ATTATAGATGGATACGAATAAGGCGTC13877931.5690618693667662No Hit
AATAGATGGATACGAATAAGGCGTC12888741.4572224011925563No Hit
ATTGTGGCGTCGCTGAACCATA12525261.4161267472818195No Hit
ATTAGATGGATACGAATAAGGCGTC11441111.2935509434210146No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATT10446121.1810557175911367No Hit
ATTTTGATGATTCATAATAACTTTTCGAATCGCA9849031.1135477281733919No Hit
ATGTGGCGTCGCTGAACCATA8711500.9849366926471443No Hit
AATATAGATGGATACGAATAAGGCGTC8633360.9761020541619869No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT7755970.8769028801091054No Hit
A6590370.7451182036527534No Hit
AATGATTAATAGGGACGGTCGGGGGC6260230.7077920256454608No Hit
ATCCAGGGGGCATGCCTGTTTGAGCGTCATT5983890.6765485652028147No Hit
AAAATATAGATGGATACGAATAAGGCGTC5553260.627860820502747No Hit
ATTGATGATTCATAATAACTTTTCGAATCGCA5488310.620517465375911No Hit
ATCTATGCCGACTAGGGATCGGGTGGTG5349930.6048719922049861No Hit
ATATATAGATGGATACGAATAAGGCGTC5301260.5993692809806119No Hit
ATTGTGGCGTCGCTGAACCATAG4978640.5628933266923927No Hit
ATGTGGCGTCGCTGAACCATAG4522930.5113699954398839No Hit
ACTATGCCGACTAGGGATCGGGTGGTG4273690.4831905061125149No Hit
ATTTGATGATTCATAATAACTTTTCGAATCGCA4220200.4771428376639475No Hit
AGCCAAGGACGTTTTCATTAATCAAGAACG3601000.4071350548381297No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT3478310.393263519187452No Hit
ATTTCCAGGGGGCATGCCTGTTTGAGCGTCATT3464400.3916908314304961No Hit
AGTGGCGTCGCTGAACCATA3445720.38957883953258543No Hit
AACTATGCCGACTAGGGATCGGGTGGTG3034170.34304831139633657No Hit
AATGATTAATAGGGACGGTCGGGGGCA2896490.32748198139075096No Hit
ATTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCA2802360.31683948688591534No Hit
AAATAGATGGATACGAATAAGGCGTC2690810.3042274581807797No Hit
ATTGACCTCAAATCAGGTAGGAGTACCCGCTGAA2672230.30212677244934605No Hit
AACCATACGCGAAACTCAGGTGCTGCAATCT2496780.28229010336538335No Hit
AATTCCAGGGGGCATGCCTGTTTGAGC2288650.25875857907672467No Hit
AGCCAAGGACGTTTTCATTAATCAAGAAC1896470.21441805538707798No Hit
ATCAATATAGATGGATACGAATAAGGCGTC1817330.2054703552371503No Hit
ATGCCAAGGACGTTTTCATTAATCAAGAACGAA1703330.19258132545332723No Hit
ATTGCCAAGGACGTTTTCATTAATCAAGAACGAA1653160.1869090217317974No Hit
ATTTCCAGGGGGCATGCCTGTTTGAGCGTCATTT1642750.18573204980153776No Hit
AGTGGCGTCGCTGAACCATAG1611350.18218191352774793No Hit
ATTTTGATGATTCATAATAACTTTTCGAATCGCAT1588210.17956566660496137No Hit
ACCAGGGGGCATGCCTGTTTGAGCGTCATT1579340.17856280963844814No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGG1579310.178559417788505No Hit
ATACTTATTCAATGAAGCGGAG1500330.16962980750494058No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAAT1468960.16608306308109386No Hit
ATTACTTATTCAATGAAGCGGAG1460030.1650734224146944No Hit
ATTGGGCCAGCATCAGTTTTGGTGGCAGGATA1459040.1649614913665717No Hit
ACCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCA1450560.1640027284493189No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT1437820.1625623228401443No Hit
AGATGGATACGAATAAGGCGTC1425630.16118410114659337No Hit
ATTTGCTCGAATATATTAGCATGGAATAATAGAAT1414540.15993024728428992No Hit
AATGGATACGAATAAGGCGTC1406260.15899409669999118No Hit
ATTGACCTCAAATCAGGTAGGAGTACCCGCTGAACT1391720.1573501800942299No Hit
ATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCA1387250.1568447944527063No Hit
ATTGACCTCAAATCAGGTAGGAGTACCCGCTGA1364870.15431447439514526No Hit
ACCTATGCCGACTAGGGATCGGGTGGTG1353270.15300295908380887No Hit
AAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGA1313100.1484612720099828No Hit
AAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTG1265900.143124761432821No Hit
AACTTTCAACAACGGATCTCTTGGTTCTCGCATCG1252050.14155885737575125No Hit
AACGCGCGCTACACTGACGGAGCCAGCG1189410.1344766746945348No Hit
AAAATACTGCCAGCTGGGACTGAGGACTGCGACG1155120.13059979020955853No Hit
ATGATGGATACGAATAAGGCGTC1139760.12886316303868556No Hit
AATGATTAATAGGGACGGTCGGGGGCAT1138940.12877045247357383No Hit
AATGATTAATAGGGACGGTCGGGGG1100680.12444471317945921No Hit
AAAGAGGTGCACAATCGACCGATCCTG1088270.12304161791965881No Hit
ATAGATGGATACGAATAAGGCGT1064350.12033718289835138No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACG1042600.11787809168959566No Hit
ATTTTTAAAACCTAAAGGTAAACCTTTA1039410.11751742497897817No Hit
ATTTCCATCTAAAGCTAAATATT1034140.11692159000563826No Hit
ATCTAAGTCAGAATCCATGCTAGAACGCGGTGATT1029010.11634158366536623No Hit
ATTACTGCCAGCTGGGACTGAGGACTGCGACG995350.11253592802919533No Hit
ATCCAGGGGGCATGCCTGTTTGAGCGTCATTT985630.11143696864762725No Hit
ATCCATCTAAAGCTAAATA973070.11001691413811132No Hit
ACCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGC971920.10988689322362537No Hit
ATTGATGATTCATAATAACTTTTCGAATCGCAT964780.10907963293716488No Hit
ATCTAAGTCAGAATCCATGCTAGAACGCGGTGATTT938620.10612193978677181No Hit
ATCGAAGAGTCGAGTTGTT904260.10223714098526163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)