FastQCFastQC Report
Mon 25 Sept 2023
SRR24451160.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24451160.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105026756
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length1-131
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
A1637259915.588979059774063No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT17064141.6247421752224738No Hit
ATATAGATGGATACGAATAAGGCGTC11948171.1376310623171109No Hit
ATTTTGATGATTCATAATAACTTTTCGAATCGCAT10517421.0014038708383985No Hit
ATTGTGGCGTCGCTGAACCATAG10404210.9906247128112764No Hit
ATTATAGATGGATACGAATAAGGCGTC9469810.9016569073122662No Hit
ATAGATGGATACGAATAAGGCGTC9196700.8756530573980595No Hit
AATATAGATGGATACGAATAAGGCGTC8824390.8402039952562184No Hit
AATAAATCATTTGTATACGACTTAGAT8793040.8372190415935535No Hit
AATAGATGGATACGAATAAGGCGTC6967940.6634442750950053No Hit
ATTGTGGCGTCGCTGAACCATA6490050.617942536471373No Hit
AAAATATAGATGGATACGAATAAGGCGTC5754570.5479146666207609No Hit
ATGTGGCGTCGCTGAACCATAG5399510.5141080430971323No Hit
ATATATAGATGGATACGAATAAGGCGTC4797250.45676456007076904No Hit
ATCTATGCCGACTAGGGATCGGGTGGTG4689820.4465357379980393No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATT4377830.4168299742591307No Hit
ATTGACCTCAAATCAGGTAGGAGTACCCGCTGAA3986260.3795470936948676No Hit
ATCAATATAGATGGATACGAATAAGGCGTC3943780.37550241007158214No Hit
ATTTTGATGATTCATAATAACTTTTCGAATCGCA3673540.34977182385791294No Hit
ATCCAGGGGGCATGCCTGTTTGAGCGTCATT3535820.33665897478543466No Hit
ACTATGCCGACTAGGGATCGGGTGGTG3266280.3109950382548234No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAAT3155170.30041582927687493No Hit
ATGTGGCGTCGCTGAACCATA3112650.29636733710027185No Hit
ATTTGCTCGAATATATTAGCATGGAATAATAGAAT3016740.2872353783830094No Hit
AT2976130.2833687446273214No Hit
AATGATTAATAGGGACGGTCGGGGGC2918810.2779110877232083No Hit
ATTAGATGGATACGAATAAGGCGTC2917780.2778130174752803No Hit
AAGATGGATACGAATAAGGCGTC2735630.26046981780528383No Hit
ATTGATGATTCATAATAACTTTTCGAATCGCAT2696090.25670506285084155No Hit
AGCCAAGGACGTTTTCATTAATCAAGAACG2685590.25570531760497295No Hit
AACTATGCCGACTAGGGATCGGGTGGTG2597840.2473503037644998No Hit
ATTTGATGATTCATAATAACTTTTCGAATCGCAT2555600.24332847146111988No Hit
ATTGTGGCGTCGCTGAACCATAGC2475120.23566566218611948No Hit
ATTGTGGCGTCGCTGAACCATAGCAGGCTAGCA2405220.22901021526362292No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT2367960.22546254784828354No Hit
ATTTGTGGCGTCGCTGAACCATAG2359990.2247036935997528No Hit
ATTTCCAGGGGGCATGCCTGTTTGAGCGTCATTT2319810.220878001792229No Hit
ATTGACCTCAAATCAGGTAGGAGTACCCGCTGA2236420.21293812026337366No Hit
ATTTCCATCTAAAGCTAAATA2216080.21100147090137678No Hit
ATTGACCTCAAATCAGGTAGGAGTACCCGCTGAACT2163830.20602654813026883No Hit
AACGCGAAACTCAGGTGCTGCAATCT1930950.183853150715233No Hit
ACACGTTCTAGCATTCAAGGTCCC1888760.17983607910350008No Hit
AACCATACGCGAAACTCAGGTGCTGCAATCT1729200.16464375991961513No Hit
ATATAGATGGATACGAATAAGGCGTCC1680880.16004302751196087No Hit
AGCCAAGGACGTTTTCATTAATCAAGAACGA1638180.1559773968454286No Hit
ACAATATAGATGGATACGAATAAGGCGTC1626340.1548500650634206No Hit
ATTGACCTCAAATCAGGTAGGAGTACCCGCTGAAC1594080.15177846681278054No Hit
ATTTGTGGCGTCGCTGAACCATA1560940.14862308038915342No Hit
ATCCAGGGGGCATGCCTGTTTGAGCGTCATTT1507000.14348724624037706No Hit
ATCACGTTCTAGCATTCAAGGTCCC1489860.1418552811437878No Hit
AATGATTAATAGGGACGGTCGGGGGCA1486610.14154583618673322No Hit
ATAGATGGATACGAATAAGGCGTCC1474620.14040422232978422No Hit
ATTGTGGCGTCGCTGAACCATAGCAGGCTAGC1465520.13953777645003146No Hit
ATTTGCTCCACACAATATAGATGGATACGAAT1445100.1375935099813994No Hit
ATCCATCTAAAGCTAAATA1420300.13523220692449076No Hit
ATTTCCATCTAAAGCTAAATATT1392300.13256621960217452No Hit
ATGTGGCGTCGCTGAACCATAGC1334990.12710951483639082No Hit
ATTATAGATGGATACGAATAAGGCGTCC1310410.12476915882272893No Hit
ATCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCT1301340.12390556935796436No Hit
ACCAGGGGGCATGCCTGTTTGAGCGTCATT1289620.12278966323590913No Hit
ATGCCAAGGACGTTTTCATTAATCAAGAACGA1287590.12259637915504121No Hit
ATTGCCGCGAAGCTACCATCCGCTGGA1279060.12178420516006418No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT1264370.12038551395417754No Hit
AACTTTCAACAACGGATCTCTTGGTTCTCGCATCG1254220.11941909354983792No Hit
AACGCGCGCTACACTGACGGAGCCAGCG1218640.1160313853738375No Hit
AATATAGATGGATACGAATAAGGCGTCC1209670.11517731729236691No Hit
ATTGACCTCAAATC1199980.11425469525117961No Hit
AAATATAGATGGATACGAATAAGGCGTC1189900.11329493981514577No Hit
ATCGAAGAGTCGAGTTGTTTGGGAATGC1150560.10954922762729147No Hit
ATGTGGCGTCGCTGAACCATAGCAGGCTAGCA1143300.10885797520014805No Hit
ATCAGGCATTGCCGCGAAGCTACCATCCGCTGGA1127220.10732693676647502No Hit
AAAATGAAAGTGAACGTAGGTTGGGGCCTC1126730.10728028198833447No Hit
AACGTTCTAGCATTCAAGGTCCC1126710.10727837771167568No Hit
AAATAGATGGATACGAATAAGGCGTC1104000.10511607156561134No Hit
ATGCCAAGGACGTTTTCATTAATCAAGAACGAA1099950.10473045554220488No Hit
AATGGCTGGAAGAGGCCAGCA1099800.10471617346726392No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGG1078830.10271953939051492No Hit

[OK]Adapter Content

Adapter graph