FastQCFastQC Report
Mon 25 Sept 2023
SRR24451167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24451167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54296679
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length6-37
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTG26074624.8022495077461365No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT22652094.17191077192769No Hit
ACGTTCTAGCATTCAAGGTC21462223.952768455691369No Hit
TAGATGGATACGAATAAGGCGTC20073333.696971963975918No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT18305373.371360889309639No Hit
AGAGGTGCACAATCGACCGATCCTGA17914023.299284657906978No Hit
AGATGGATACGAATAAGGCGTC16900863.1126876102311893No Hit
AAGAGGTGCACAATCGACCGATCCTG16162672.9767327021971273No Hit
AAGAGGTGCACAATCGACCGATCCTGA11721882.15885763473674No Hit
TATAGATGGATACGAATAAGGCGTC9994221.8406687451363277No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA8296131.5279258608063304No Hit
TTGCCCGAGTTGTAATTTG7569821.394158931893422No Hit
TGCCCGAGTTGTAATTTG6945081.2790984877730736No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAA6484051.1941890589662032No Hit
TTAGATGGATACGAATAAGGCGTC5656381.0417543216593412No Hit
ATAGATGGATACGAATAAGGCGTC5358070.9868135765725193No Hit
TGTGGCGTCGCTGAACCATA4979620.9171131810842427No Hit
ATAAATCATTTGTATACGACTTAGAT4951130.9118660830066605No Hit
CACGTTCTAGCATTCAAGGTC4283760.7889543299692418No Hit
TTATAGATGGATACGAATAAGGCGTC3867710.7123290173971782No Hit
TTGTGGCGTCGCTGAACCATA3695740.6806567304051875No Hit
TCACGTTCTAGCATTCAAGGTC3343810.6158406115409011No Hit
GTGCCCGAGTTGTAATTT3235900.5959664678570856No Hit
ATATAGATGGATACGAATAAGGCGTC3016560.5555698903794835No Hit
GATGGATACGAATAAGGCGTC2622350.4829669232624706No Hit
TACTTATTCAATGAAGCGGAG2488020.45822692028733475No Hit
TTACTTATTCAATGAAGCGGAG2485100.45768913417338103No Hit
GTGCCCGAGTTGTAATTTG2459480.45297061354341767No Hit
TGTGGCGTCGCTGAACCATAG2392080.440557331324076No Hit
GAGGTGCACAATCGACCGATCCTG2265580.4172594054969734No Hit
AAAGAGGTGCACAATCGACCGATCCTG2006880.3696137658805983No Hit
ACGTTCTAGCATTCAAGGTCC1968050.36246231560497466No Hit
GTGGCGTCGCTGAACCATA1910720.3519036587854664No Hit
AAATATAGATGGATACGAATAAGGCGTC1852370.34115714517272777No Hit
TGATGGATACGAATAAGGCGTC1824760.33607211962263844No Hit
TAAGAGGTGCACAATCGACCGATCCTG1731860.31896241757253696No Hit
TATATAGATGGATACGAATAAGGCGTC1730530.31871746704803067No Hit
TAGAGGTGCACAATCGACCGATCCTG1705790.3141610189455602No Hit
TTTGCTCGAATATATTAGCATG1568320.28884271172459736No Hit
GAGGTGCACAATCGACCGATCCTGA1557760.2868978413946827No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA1549370.28535262718369936No Hit
AGAGGTGCACAATCGACCGATCCT1524870.280840380679636No Hit
TTGTGGCGTCGCTGAACCATAG1511630.27840192583417483No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA1432180.26376935502814086No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA1391210.25622377383338674No Hit
AAAGAGGTGCACAATCGACCGATCCTGA1362310.25090116469185897No Hit
TATGACTCTTAATCATAAGGTTAGGGGT1268350.23359623891545928No Hit
TGTGCCCGAGTTGTAATTT1263260.23265879668257425No Hit
TTTTACTTATTCAATGAAGCGGAG1247160.2296936061227612No Hit
ATGGATACGAATAAGGCGTC1246420.2295573178610058No Hit
TAAGAGGTGCACAATCGACCGATCCTGA1178080.2169709127145695No Hit
TAGAGGTGCACAATCGACCGATCCTGA1130160.208145326899275No Hit
TTTACTTATTCAATGAAGCGGAG1127030.20756886438671507No Hit
TTTTCTTTACTTATTCAATGAAGCGGA1123580.20693346640961227No Hit
AAGAGGTGCACAATCGACCGATCCT1024160.1886229542694499No Hit
ACGAATAAGGCGTC1008760.18578668503832435No Hit
TTGCTCGAATATATTAGCATG976600.1798636708517661No Hit
AATAGATGGATACGAATAAGGCGTC974270.17943454700056333No Hit
TGTGCCCGAGTTGTAATTTG973960.17937745326928745No Hit
TCTGACTTCTAATCAGAAGATTATGGGT873560.1608864512689625No Hit
ACGTTCTAGCATTCAAGGTCCC821100.15122471855046604No Hit
TAGATGGATACGAATAAGGCGT807610.14874022037333073No Hit
GTGGCGTCGCTGAACCATAG785800.14472340011808088No Hit
TTTCTTTACTTATTCAATGAAGCGGA781850.14399591547762985No Hit
AATATAGATGGATACGAATAAGGCGTC777670.14322607097203865No Hit
CATTCAAGGTC771290.1420510451477152No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGA758860.13976177069687815No Hit
ATAAGGCGTC724510.13343541692485464No Hit
AGATGGATACGAATAAGGCGT698800.1287003206954886No Hit
TCTGACTTCTAATCAGA688660.12683280316278644No Hit
TGAGGTGCACAATCGACCGATCCTG670890.12356004314739029No Hit
TTTTCTTTACTTATTCAATGAAGCGGAG667530.12294122076969016No Hit
TTGGCGTCGCTGAACCATAG648710.11947507876126273No Hit
ATAGAAT642870.11839950653335539No Hit
AAAGATTGGAAATCTTTTG639810.11783593615366421No Hit
ACGTTCTAGCATTCAAGGT633790.11672721272695151No Hit
TGGCGTCGCTGAACCATAG624750.11506228585361547No Hit
TTGCTCGAATATATTAGCATGGAATAATAGA623780.11488363772671989No Hit
CGTTCTAGCATTCAAGGTC619180.11403644042391617No Hit
TAAGGCGTC616980.11363125910518394No Hit
CTGCCCGAGTTGTAATTTG600600.11061450001389588No Hit
TGGGAGAGGTCTCCGGT593720.1093473875262242No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAT551630.10159553220557008No Hit
AGAGGTGCACAATCGACCGATC547540.10084226329938152No Hit
TTTCTTTACTTATTCAATGAAGCGGAG545920.10054390251013326No Hit

[OK]Adapter Content

Adapter graph