FastQCFastQC Report
Mon 25 Sept 2023
SRR24451168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24451168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81308533
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length6-37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAAT35780844.400625454649391No Hit
TAGATGGATACGAATAAGGCGTC33555774.126967830055426No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT32373453.9815562777402467No Hit
AGATGGATACGAATAAGGCGTC32310413.973803093950791No Hit
AGAGGTGCACAATCGACCGATCCTG20874382.56730495924702No Hit
TATAGATGGATACGAATAAGGCGTC17120642.105638777174838No Hit
AAGAGGTGCACAATCGACCGATCCTG15740041.9358411004660483No Hit
AGAGGTGCACAATCGACCGATCCTGA14697781.8076552924648142No Hit
AAGAGGTGCACAATCGACCGATCCTGA11646681.4324056246347476No Hit
ACGTTCTAGCATTCAAGGTC9493331.1675687224611468No Hit
ATAGATGGATACGAATAAGGCGTC9121011.1217777105878912No Hit
TGTGGCGTCGCTGAACCATA8978281.104223587455452No Hit
TTAGATGGATACGAATAAGGCGTC8861541.0898659307996617No Hit
TTGCCCGAGTTGTAATTTG8185601.0067332047424837No Hit
TGCCCGAGTTGTAATTTG7964300.9795158891871779No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA7424760.9131587701871341No Hit
ATAAATCATTTGTATACGACTTAGAT6628830.8152686754291828No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAA6331330.7786796497730442No Hit
TTGTGGCGTCGCTGAACCATA6258710.7697482378633003No Hit
TTATAGATGGATACGAATAAGGCGTC6112550.7517722647880021No Hit
ACGAATAAGGCGTC5911220.7270110260137149No Hit
AAGGCGTC5754030.707678491751905No Hit
GATGGATACGAATAAGGCGTC5293530.6510423696858484No Hit
TAAGGCGTC5168360.6356479214795329No Hit
CACGTTCTAGCATTCAAGGTC5028580.6184566138956166No Hit
ATATAGATGGATACGAATAAGGCGTC4878370.5999825381181088No Hit
TCACGTTCTAGCATTCAAGGTC4381910.5389237560097168No Hit
TATGACTCTTAATCATAAGGTTAGGGGT4125130.5073428148064115No Hit
TGATGGATACGAATAAGGCGTC3230740.3973432899102976No Hit
GTGGCGTCGCTGAACCATA3081530.37899220245432297No Hit
TTTTCTTTACTTATTCAATGAAGCGGA3079080.37869088106656656No Hit
GTGCCCGAGTTGTAATTTG3055570.37579942562732005No Hit
AAATATAGATGGATACGAATAAGGCGTC3027560.37235452274117403No Hit
GTGCCCGAGTTGTAATTT2948440.36262368674146417No Hit
TCTGACTTCTAATCAGAAGATTATGGGT2903020.3570375571774244No Hit
TATATAGATGGATACGAATAAGGCGTC2876650.3537943551385929No Hit
AGAGGTGCAC2535150.31179384333499166No Hit
TCTGACTTCTAATCAGA2460460.302607845599674No Hit
TTACTTATTCAATGAAGCGGAG2395980.2946775586272108No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA2366660.29107154103985616No Hit
TTTTACTTATTCAATGAAGCGGAG2346560.28859947577703804No Hit
AAGAGGTGCAC2201910.270809215067255No Hit
TTTCTTTACTTATTCAATGAAGCGGA2173570.267323726035003No Hit
TGTGGCGTCGCTGAACCATAG2117860.2604720466423862No Hit
TGGGAGAGGTCTCCGGT2086980.25667416727343983No Hit
ATGGATACGAATAAGGCGTC1995890.24547116106497704No Hit
AAAGAGGTGCACAATCGACCGATCCTG1914750.23549188865576998No Hit
AAAGATTGGAAATCTTTTG1877530.2309142633283028No Hit
GAGGTGCACAATCGACCGATCCTG1836820.22590740875868465No Hit
TTGCTCGAATATATTAGC1674100.20589474907879596No Hit
TAAGAGGTGCACAATCGACCGATCCTG1671960.20563155407071482No Hit
TTTGCTCGAATATATTAGCATGGAAT1650370.20297623620881217No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA1611090.19814525493898655No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA1603260.19718225638138126No Hit
AATAGATGGATACGAATAAGGCGTC1579270.19423176654779886No Hit
AATATAGATGGATACGAATAAGGCGTC1557940.19160842564949487No Hit
TTTACTTATTCAATGAAGCGGAG1553580.19107219656760993No Hit
TATGACTCTTAATCATA1504530.18503961939640456No Hit
TTTTCTTTACTTATTCAATGAAGCGGAG1460830.179665029745402No Hit
ACGTTCTAGCATTCAAGGTCC1448720.17817564117163445No Hit
AAATCGACCGATCCTG1446300.17787800943352403No Hit
TTGCTCGAATATATT1443510.1775348720164463No Hit
ATGGAATAATAGAAT1424070.17514397904584011No Hit
ATTAGCATGGAATAATAGAAT1375950.16922578101366065No Hit
TTGTGGCGTCGCTGAACCATAG1346840.16564559097382803No Hit
AAAGAGGTGCACAATCGACCGATCCTGA1321450.1625229174900991No Hit
TAGAGGTGCACAATCGACCGATCCTG1309680.16107534494565287No Hit
TACTTATTCAATGAAGCGGAG1306620.16069900068176116No Hit
GAGGTGCACAATCGACCGATCCTGA1292930.15901529055997113No Hit
AAAGGCGTC1268400.15599838703276075No Hit
TTGCTCGAATATATTAGCATGGAAT1232630.15159909477151678No Hit
TTTGCTCGAAT1193860.1468308375456731No Hit
TTTCTTTACTTATTCAATGAAGCGGAG1178200.14490484043046256No Hit
TTTGCTCGAATATATT1166510.143467106951739No Hit
TGTGCCCGAGTTGTAATTTG1161430.14284232627835014No Hit
TAAGAGGTGCACAATCGACCGATCCTGA1158520.14248443026268842No Hit
TTGCTCGAAT1145420.14087328325060297No Hit
TGTGCCCGAGTTGTAATTT1117130.13739394363442764No Hit
AACTTTTCGAATCGCAT1062640.13069230999408143No Hit
AATCGACCGATCCTG1034040.12717484399823079No Hit
TCAGTCTCATAATCTGA990390.12180640376330489No Hit
TTTGCTCGAATATATTAGCATGGAATAAT988830.12161454198171304No Hit
ACAATCGACCGATCCTG955870.11756084690397747No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAT953810.117307490961619No Hit
ATCTGTTG947380.11651667605416026No Hit
TGCGACTGTTAATCGCA932740.11471612702691365No Hit
CATAGATGGATACGAATAAGGCGTC924070.11364981827922047No Hit
ATGGGCGCTTGTCGCGTGCC892500.1097670769684161No Hit
ATAAGGCGTC885230.10887295187086944No Hit
TAGAGGTGCACAATCGACCGATCCTGA872250.107276563457368No Hit
TGGGAGAGGTCTCCGG857220.10542804898472341No Hit
ATGCCTTGCTAAGGCATTG828320.10187368649241278No Hit
AGAGGTGCACAATCGACCGATC826850.10169289365975892No Hit
ATATTAGCATGGAATAATAGAAT814440.10016660858953143No Hit

[OK]Adapter Content

Adapter graph