FastQCFastQC Report
Mon 25 Sept 2023
SRR24451169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR24451169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99721010
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length6-37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAAT59797865.996515679093102No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT50908335.105075650557491No Hit
AGAGGTGCACAATCGACCGATCCTG33184513.3277350480104446No Hit
AAGAGGTGCACAATCGACCGATCCTG27995062.80733819282416No Hit
AGAGGTGCACAATCGACCGATCCTGA24656842.4725822572394724No Hit
AAGAGGTGCACAATCGACCGATCCTGA22722112.27856797679847No Hit
TAGATGGATACGAATAAGGCGTC22593762.2656970682507125No Hit
AGATGGATACGAATAAGGCGTC19344241.9398359483121963No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA17703171.7752698252855643No Hit
ACGTTCTAGCATTCAAGGTC15240651.5283288847555796No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAA13085301.3121908813398502No Hit
TTGCCCGAGTTGTAATTTG12720901.2756489329580596No Hit
TATAGATGGATACGAATAAGGCGTC12289261.2323641728057106No Hit
TGCCCGAGTTGTAATTTG11593641.1626075588283753No Hit
ATAAATCATTTGTATACGACTTAGAT8648670.8672866430053207No Hit
TGTGGCGTCGCTGAACCATA8430280.8453865439188792No Hit
CACGTTCTAGCATTCAAGGTC7998180.8020556550720856No Hit
TCACGTTCTAGCATTCAAGGTC6905680.6925000057660868No Hit
TTAGATGGATACGAATAAGGCGTC6600650.6619116673607698No Hit
ATAGATGGATACGAATAAGGCGTC6404530.6422447987640719No Hit
TTGTGGCGTCGCTGAACCATA6220220.6237622342573546No Hit
GTGCCCGAGTTGTAATTT5450000.5465247493983465No Hit
GTGCCCGAGTTGTAATTTG4874330.4887966938963013No Hit
TTATAGATGGATACGAATAAGGCGTC4846380.4859938743099373No Hit
ATATAGATGGATACGAATAAGGCGTC3966050.3977145839176719No Hit
TGTGGCGTCGCTGAACCATAG3948930.3959977942461674No Hit
TATGACTCTTAATCATAAGGTTAGGGGT3755810.3766317649610649No Hit
AAAGAGGTGCACAATCGACCGATCCTG3423610.3433188251903987No Hit
TAAGAGGTGCACAATCGACCGATCCTG2960670.2968953082204041No Hit
GAGGTGCACAATCGACCGATCCTG2907530.2915664412143439No Hit
GTGGCGTCGCTGAACCATA2892790.2900883173967051No Hit
GATGGATACGAATAAGGCGTC2877620.28856707327773756No Hit
TCTGACTTCTAATCAGAAGATTATGGGT2672040.26795155805180876No Hit
TTGTGGCGTCGCTGAACCATAG2610950.2618254668700207No Hit
AAAGAGGTGCACAATCGACCGATCCTGA2595520.2602781500107149No Hit
TTACTTATTCAATGAAGCGGAG2447620.2454467719490607No Hit
AAATATAGATGGATACGAATAAGGCGTC2391970.23986620271896567No Hit
TTTTACTTATTCAATGAAGCGGAG2378950.23856056010664153No Hit
TATATAGATGGATACGAATAAGGCGTC2283280.2289667944598636No Hit
TAAGAGGTGCACAATCGACCGATCCTGA2246000.22522836461443785No Hit
TTTTCTTTACTTATTCAATGAAGCGGA2213480.22196726647674345No Hit
TGTGCCCGAGTTGTAATTT2170680.21767529229798216No Hit
ACGTTCTAGCATTCAAGGTCC2168560.21746269918445474No Hit
TGGGAGAGGTCTCCGGT2116500.21224213433056882No Hit
GAGGTGCACAATCGACCGATCCTGA2090900.20967497220495462No Hit
TAGAGGTGCACAATCGACCGATCCTG2037140.20428393174116466No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA2024980.2030645297314979No Hit
TGTGCCCGAGTTGTAATTTG1919490.19248601673809762No Hit
TGATGGATACGAATAAGGCGTC1859930.1865133536052232No Hit
TTTTCTTTACTTATTCAATGAAGCGGAG1686580.1691298553835345No Hit
ACGAATAAGGCGTC1672130.16768081269934992No Hit
AAAGATTGGAAATCTTTTG1670140.16748125595599161No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGA1652220.16568424246806163No Hit
TACTTATTCAATGAAGCGGAG1543570.15478884539978083No Hit
ATGGAATAATAGAAT1502670.15068740278502996No Hit
AAGGCGTC1500550.15047480967150254No Hit
TTTACTTATTCAATGAAGCGGAG1491670.14958432530918006No Hit
TCTGACTTCTAATCAGA1463490.14675844137559377No Hit
TAGAGGTGCACAATCGACCGATCCTGA1457960.14620389424455288No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA1430790.1434792928792037No Hit
ATAGAAT1429680.14336798233391337No Hit
TTTCTTTACTTATTCAATGAAGCGGA1426090.1430079779577042No Hit
ATGGATACGAATAAGGCGTC1395850.13997551769682237No Hit
TGCCCGAGTTGTAATTT1352460.13562437845344727No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA1343830.13475896403375778No Hit
AGAGGTGCACAATCGACCGATCCT1343590.13473489688883017No Hit
CATTCAAGGTC1339360.13431071345948062No Hit
TTTCTTTACTTATTCAATGAAGCGGAG1275120.12786874100051734No Hit
TGCGACTGTTAATCGCA1246650.12501377593347682No Hit
GTGGCGTCGCTGAACCATAG1231320.12347648705122422No Hit
TTGCTCGAATATATTAGCATGGAATAATAGA1229690.12331303102525737No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAT1194660.11980023066352817No Hit
AAGAGGTGCACAATCGACCGATCCT1185210.1188525868320026No Hit
TATGACTCTTAATCATA1182080.11853871115023805No Hit
TGGGAGAGGTCTCCGG1164350.11676075081870911No Hit
ATGGGCGCTTGTCGCGTGCC1140600.1143791062685787No Hit
AATAGATGGATACGAATAAGGCGTC1134390.11375636889357618No Hit
TAAGGCGTC1086730.10897703503003027No Hit
ATTAGCATGGAATAATAGAAT1068910.1071900495191535No Hit
CACGTTCTAGCATTCAAGGTCC1060240.10632062390864272No Hit
TTGCCCGAGTTGTAATTT1057160.10601176221540476No Hit
ATCTGTTG1050480.10534189334825228No Hit
AATATAGATGGATACGAATAAGGCGTC1047750.10506812957470045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)